{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:47:51Z","timestamp":1787240871601,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T00:00:00Z","timestamp":1490313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Mechanical forces, hypoxia, and oxidative stress contribute to skin renewal, perfusion, and wound healing, but how are they regulating subcutaneous adipose\u2010derived stem cells (ASCs) in the inflammatory microenvironment associated to skin repair and disorders is unknown. In this study, ASCs were isolated from lipoaspirate samples from plastic surgery patients, primary cultured and their differentiation and secretion of a panel of cytokines with pronounced effects on skin repair and angiogenesis were studied under mechanical stimulation by intermittent fluid flow, 1% hypoxia and oxidative stress by glutathione (GSH) depletion with buthionine sulfoximine (BSO) treatment. Mechanical action of fluid flow did not alter mesenchymal phenotype of CD90\n                      <jats:sup>+<\/jats:sup>\n                      \/CD29\n                      <jats:sup>+<\/jats:sup>\n                      \/CD44\n                      <jats:sup>+<\/jats:sup>\n                      \/CD34\n                      <jats:sup>\u2212<\/jats:sup>\n                      \/CD106\n                      <jats:sup>\u2212<\/jats:sup>\n                      \/CD45\n                      <jats:sup>\u2212<\/jats:sup>\n                      ASCs; however, it remarkably induced ASC secretion of human umbilical vein endothelial cell (HUVEC) migration\u2010stimulating factors. Multiplex Luminex assay further confirmed an increased secretion of VEGF, G\u2010CSF, HGF, Leptin, IL\u20108, PDGF\u2010BB, Angiopoietin\u20102, and Follistatin from mechanically\u2010stimulated ASCs via cyclooxygenase\u20102. Consistent with this mechanism, GSH depletion and hypoxia also increased ASC secretion of VEGF, IL\u20108, leptin, Angiopoitein\u20102, and PDGF\u2010BB. However, mechanical action of fluid flow abrogated VEGF and HUVEC migration\u2010stimulating activity from GSH\u2010depleted and hypoxic ASCs. Conversely, GSH depletion and hypoxia abrogated VEGF and HUVEC migration\u2010stimulating activity from mechano\u2010stimulated ASCs. Although mechanical action of fluid flow, hypoxia, and GSH\u2010depletion had independent proangiogenic\u2010stimulating activity on ASCs, mechanical stimulation had opposite effects on proangiogenic factor secretion from ASCs with and without oxidative stress. These data uncover the role of hypoxia and endogenous redox balance during the proangiogenic response of ASCs and other mesenchymal\u2010derived cell types to mechanical action of interstitial fluid flow. J. Cell. Physiol. 232: 2158\u20132167, 2017. \u00a9 2016 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.25712","type":"journal-article","created":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T07:20:34Z","timestamp":1481095234000},"page":"2158-2167","source":"Crossref","is-referenced-by-count":16,"title":["Opposite Effects of Mechanical Action of Fluid Flow on Proangiogenic Factor Secretion From Human Adipose\u2010Derived Stem Cells With and Without Oxidative Stress"],"prefix":"10.1002","volume":"232","author":[{"given":"Beatriz","family":"Bravo","sequence":"first","affiliation":[{"name":"Institute of Applied Molecular Medicine (IMMA) CEU\u2010San Pablo University School of Medicine Boadilla del Monte Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cira","family":"Garc\u00eda de Durango","sequence":"additional","affiliation":[{"name":"Institute of Applied Molecular Medicine (IMMA) CEU\u2010San Pablo 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