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Although many findings have been clarified in the territory of BBB and BNB, few have so far examined the TJs of capillaries in the skeletal muscle. In addition, no in vitro human skeletal muscle microvasculature models have been reported thus far. We newly established a new human skeletal muscle microvascular endothelial cell (HSMMEC) line. HSMMECs were isolated from human skeletal muscle and were infected with retroviruses harboring temperature\u2010sensitive SV40 T antigen and telomerase genes. This cell line, termed TSM15, showed a spindle fiber\u2010shaped morphology, an immunoreactivity to anti\u2010factor VIII and anti\u2010VE\u2010cadherin antibodies, and a temperature\u2010sensitive growth. TSM15 cells grew stably for more than 40 passages when they were cultured at 33\u00b0C, thereby retaining their spindle fiber\u2010shaped morphology and contact inhibition at confluence. The cells expressed tight junctional molecules such as claudin\u20105, occludin, and zonula occludens\u20101, as well as transporters such as a glucose transporter 1. The transendothelial electrical resistance of TSM15 was as high as those of the human brain microvascular endothelial cell line. This novel cell line might facilitate the analyses of the pathophysiology of inflammatory myopathy, such as dermatomyositis, and can improve our understanding of the physiological and biochemical properties of the microvasculature in human skeletal muscle.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25772","type":"journal-article","created":{"date-parts":[[2017,1,7]],"date-time":"2017-01-07T10:28:41Z","timestamp":1483784921000},"page":"3286-3295","source":"Crossref","is-referenced-by-count":10,"title":["Establishment of a new conditionally immortalized human skeletal muscle microvascular endothelial cell line"],"prefix":"10.1002","volume":"232","author":[{"given":"Hironori","family":"Sano","sequence":"first","affiliation":[{"name":"Department of Neurology and Clinical Neuroscience Yamaguchi University Graduate School of Medicine Ube Yamaguchi 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