{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:26:42Z","timestamp":1785130002845,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T00:00:00Z","timestamp":1487635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["67037"],"award-info":[{"award-number":["67037"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>An upregulation of Egr\u20101 expression has been reported in models of atherosclerosis and intimal hyperplasia and, various vasoactive peptides and growth promoting stimuli have been shown to induce the expression of Egr\u20101 in vascular smooth muscle cells (VSMC). Angiotensin\u2010II (Ang\u2010II) is a key vasoactive peptide that has been implicated in the pathogenesis of vascular diseases. Ang\u2010II elevates intracellular Ca<jats:sup>2+<\/jats:sup>through activation of the store\u2010operated calcium entry (SOCE) involving an inositol\u20103\u2010phosphate receptor (IP3R)\u2010coupled depletion of endoplasmic reticular Ca<jats:sup>2+<\/jats:sup>and a subsequent activation of the stromal interaction molecule 1 (STIM\u20101)\/Orai\u20101 complex. However, the involvement of IP3R\/STIM\u20101\/Orai\u20101\u2010Ca<jats:sup>2+<\/jats:sup>\u2010dependent signaling in Egr\u20101 expression in VSMC remains unexplored. Therefore, in the present studies, we have examined the role of Ca<jats:sup>2+<\/jats:sup>signaling in Ang\u2010II\u2010induced Egr\u20101 expression in VSMC and investigated the contribution of STIM\u20101 or Orai\u20101 in mediating this response. 2\u2010aminoethoxydiphenyl borate (2\u2010APB), a dual non\u2010competitive antagonist of IP3R and inhibitor of SOCE, decreased Ang\u2010II\u2010induced Ca<jats:sup>2+<\/jats:sup>release and attenuated Ang\u2010II\u2010induced enhanced expression of Egr\u20101 protein and mRNA levels. Egr\u20101 upregulation was also suppressed following blockade of calmodulin and CaMKII. Furthermore, RNA interference\u2010mediated depletion of STIM\u20101 or Orai\u20101 attenuated Ang\u2010II\u2010induced Egr\u20101 expression as well as Ang\u2010II\u2010induced phosphorylation of ERK1\/2 and CREB. In addition, siRNA\u2010induced silencing of CREB resulted in a reduction in the expression of Egr\u20101 stimulated by Ang\u2010II. In summary, our data demonstrate that Ang\u2010II\u2010induced Egr\u20101 expression is mediated by STIM\u20101\/Orai\u20101\/Ca<jats:sup>2+<\/jats:sup>\u2010dependent signaling pathways in A\u201010 VSMC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25810","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T09:06:35Z","timestamp":1484903195000},"page":"3496-3509","source":"Crossref","is-referenced-by-count":42,"title":["STIM\u20101 and ORAI\u20101 channel mediate angiotensin\u2010II\u2010induced expression of Egr\u20101 in vascular smooth muscle cells"],"prefix":"10.1002","volume":"232","author":[{"given":"Estelle R.","family":"Simo\u2010Cheyou","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Signaling Montreal Diabetes Research Center Quebec Canada"},{"name":"CHUM\u2010Research Center (CRCHUM) Quebec Canada"},{"name":"Faculty of Medicine, Department of Nutrition University of Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju Jing","family":"Tan","sequence":"additional","affiliation":[{"name":"CHUM\u2010Research Center (CRCHUM) Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryszard","family":"Grygorczyk","sequence":"additional","affiliation":[{"name":"CHUM\u2010Research Center (CRCHUM) Quebec Canada"},{"name":"Faculty of Medicine, Department of Medicine University of Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok K.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Signaling Montreal Diabetes Research Center Quebec Canada"},{"name":"CHUM\u2010Research Center (CRCHUM) Quebec Canada"},{"name":"Faculty of Medicine, Department of Nutrition University of Montreal Quebec Canada"},{"name":"Faculty of Medicine, Department of Medicine University of Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.496802"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2006.02022.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00325.2009"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01097.2006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2013.10.002"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000218176"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570161112666140226123139"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.115.06114"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000214980.90567.b5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(95)00088-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110430200"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.108.124404"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2011007500133"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(01)00497-X"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr307"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.155143"},{"issue":"2","key":"e_1_2_7_18_1","first-page":"195","article-title":"Ip3 dependent ca2+ signals in muscle cells are involved in regulation of gene expression","volume":"35","author":"Jaimovich E.","year":"2002","journal-title":"Biology Reseasrch"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000200177.53882.c3"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01014.2009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.08.051"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813307106"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.490870"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5458.1647"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.202697"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2008.07.002"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.4.418"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406063200"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079\u2010013\u20100215\u20105"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-014-0431-2"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/351233a0"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.2174\/157016106775203126"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302153"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(03)00026-5"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-131128"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200502019"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-10-40"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65341-2"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.68.1.821"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.03749.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21085"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.07.096"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2010.02.005"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2005.030080"},{"issue":"4","key":"e_1_2_7_45_1","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/S0031-6997(24)01471-6","article-title":"Signal transduction mechanisms mediating the physiological and pathophysiological actions of angiotensin ii in vascular smooth muscle cells","volume":"52","author":"Touyz R. M.","year":"2000","journal-title":"Pharmacological Reviews"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003147200"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq213"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01191.2004"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12684"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1118947109"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25260"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04147"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.246777"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01124.2010"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.25810","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.25810","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T17:46:53Z","timestamp":1749923213000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.25810"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,21]]},"references-count":53,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1002\/jcp.25810"],"URL":"https:\/\/doi.org\/10.1002\/jcp.25810","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,21]]}}}