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The primary function of fibroblasts is to synthesize structural proteins, such as collagen, extracellular matrix proteins, and other proteins that support the structural integrity of the skin and are associated with younger, firmer, and more elastic skin that is better able to resist and recover from injury. At sub\u2010nanomolar concentrations (0.03\u20130.3\u2009nM), bryostatin\u20101 and its synthetic analog, picolog (0.1\u201310\u2009nM) sustained the survival and activation of human dermal fibroblasts cultured under the stressful condition of prolonged serum deprivation. Bryostatin\u20101 treatment stabilized human skin equivalents (HSEs), a bioengineered combination of primary human skin cells (keratinocytes and dermal fibroblasts) on an extracellular matrix composed of mainly collagen. Fibroblasts activated by bryostatin\u20101 protected the structural integrity of HSEs. Bryostatin\u20101 and picolog prolonged activation of Erk in fibroblasts to promote cell survival. Chronic stress promotes the progression of apoptosis. Dermal fibroblasts constitutively express all components of Fas associated apoptosis, including caspase\u20108, an initiator enzyme of apoptosis. Prolong bryostatin\u20101 treatment reduced apoptosis by decreasing caspase\u20108 and protected dermal fibroblasts. Our data suggest that bryostatin\u20101 and picolog could be useful in anti\u2010aging skincare, and could have applications in tissue engineering and regenerative medicine.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26043","type":"journal-article","created":{"date-parts":[[2017,6,7]],"date-time":"2017-06-07T11:46:35Z","timestamp":1496835995000},"page":"1523-1534","source":"Crossref","is-referenced-by-count":6,"title":["Bryostatin and its synthetic analog, picolog rescue dermal fibroblasts from prolonged stress and contribute to survival and rejuvenation of human skin equivalents"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0737-3884","authenticated-orcid":false,"given":"Tapan K.","family":"Khan","sequence":"first","affiliation":[{"name":"Center for Neurodegenerative Diseases Blanchette Rockefeller Neurosciences Institute at West Virginia University Morgantown West Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul 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