{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:59:42Z","timestamp":1787500782978,"version":"build-2736575974"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T00:00:00Z","timestamp":1505174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004572","name":"National Health and Family Planning Commission of the People's Republic of China","doi-asserted-by":"publisher","award":["2017\u2010169"],"award-info":[{"award-number":["2017\u2010169"]}],"id":[{"id":"10.13039\/501100004572","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Tumor metastasis is responsible for 90% of all cancer\u2010related deaths. Epithelial to mesenchymal transition (EMT) is an important prerequisite for tumor metastasis. One of the important mediators of EMT and cancer progression in ovarian cancer is the vimentin protein. The objective of the current study was to evaluate the molecular mechanism that regulates vimentin expression in ovarian cancer cells. Vimentin was robustly induced in the ovarian cancer cell line SKOV\u20103 compared to normal ovarian epithelial cell line Moody and the induction was not due to transcriptional upregulation. Treatment with the proteasomal inhibitor MG\u2010132 revealed that vimentin is actively degraded by the proteasome in Moody cells and stabilized in the SKOV\u20103 cell line. Mass spectrometric analysis of vimentin immunoprecipitate of MG\u2010132 treated Moody cells revealed candidate ubiquitin ligases associated with vimentin. RNAi mediated silencing of the candidate ubiquitin in Moody cells and concurrent overexpression of the candidate ubiquitin ligases in SKOV\u20103 confirmed that TRIM56 is the ubiquitin ligase that is degrading vimentin in Moody cells. RNAi mediated silencing of\n                      <jats:italic>TRIM56<\/jats:italic>\n                      in Moody cells and ectopic overexpression of\n                      <jats:italic>TRIM56<\/jats:italic>\n                      in SKOV\u20103 cells, respectively, significantly up\u2010 and down\u2010regulated in vitro migration and invasion in these cells. Analysis of\n                      <jats:italic>TRIM56<\/jats:italic>\n                      transcript level and vimentin protein expression in 25 patients with ovarian carcinoma confirmed an inverse correlation between\n                      <jats:italic>TRIM56<\/jats:italic>\n                      and vimentin expression. Cumulatively, our data reveals for the first time a novel post\u2010translational regulatory mechanism of regulating vimentin expression, EMT, and metastatic progression in ovarian cancer cells.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26114","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T16:09:39Z","timestamp":1501776579000},"page":"2420-2425","source":"Crossref","is-referenced-by-count":55,"title":["The ubiquitin ligase TRIM56 inhibits ovarian cancer progression by targeting vimentin"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4297-1337","authenticated-orcid":false,"given":"Lei","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing Daqing Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing Daqing Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010jie","family":"Su","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing Daqing Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing Daqing Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3931"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041842"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6510"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-262"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15748"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-14-0234"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605626"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084941"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-4848-x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2013.09.007"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-3175"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.10.013"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v6.i4.441"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02546-10"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0364"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.128"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16064"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26114","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T10:25:32Z","timestamp":1696674332000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,12]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1002\/jcp.26114"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26114","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,12]]}}}