{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:26:36Z","timestamp":1773098796020,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005145","name":"Basic Research Program of Jiangsu Province","doi-asserted-by":"publisher","award":["KYCX17_1821"],"award-info":[{"award-number":["KYCX17_1821"]}],"id":[{"id":"10.13039\/501100005145","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005065","name":"Innovative Team of Jiangsu Province","doi-asserted-by":"publisher","award":["CXTDB2017002"],"award-info":[{"award-number":["CXTDB2017002"]}],"id":[{"id":"10.13039\/501100005065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270630"],"award-info":[{"award-number":["81270630"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M601748"],"award-info":[{"award-number":["2016M601748"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Previous study has revealed that <jats:italic>H19<\/jats:italic> expression is required for efficient tumor growth induced by <jats:italic>BCR\u2010ABL<\/jats:italic> in chronic myeloid leukemia (CML). Herein, we further determined <jats:italic>H19<\/jats:italic> expression and its clinical implication in patients with CML. <jats:italic>H19<\/jats:italic> expression and methylation were detected by real\u2010time quantitative PCR and real\u2010time quantitative methylation\u2010specific PCR, and then clinical implication of <jats:italic>H19<\/jats:italic> expression was further analyzed. <jats:italic>H19<\/jats:italic> expression was significantly up\u2010regulated in CML patients (<jats:italic>p\u2009<\/jats:italic>&lt;\u20090.001). <jats:italic>H19<\/jats:italic> expression with an area under receiver operating characteristic curve value of 0.824 might serve as a promising biomarker in distinguishing CML patients from controls. The patients with high <jats:italic>H19<\/jats:italic> expression had a tendency of higher white blood cells and <jats:italic>BCR\u2010ABL<\/jats:italic> transcript than those with low <jats:italic>H19<\/jats:italic> expression. <jats:italic>H19<\/jats:italic> overexpression occurred with the higher frequency in blast crisis stage (11\/11, 100%), lower in accelerated phase (3\/5, 60%), and chronic phase (42\/62, 66%) stages. Moreover, paired patients during disease progression with increased <jats:italic>BCR\u2010ABL<\/jats:italic> transcript also showed a significant upregulation of <jats:italic>H19<\/jats:italic> expression. Meanwhile, <jats:italic>H19<\/jats:italic> expression was decreased in follow\u2010up patients who achieved complete molecular remission after tyrosine kinase inhibitors\u2010based therapy. Epigenetic studies showed that <jats:italic>H19<\/jats:italic> differentially methylated region\/imprinting control region (DMR\/ICR) was hypomethylated and associated with <jats:italic>H19<\/jats:italic> expression in CML patients. Moreover, demethylation of <jats:italic>H19<\/jats:italic> DMR\/ICR reactivated <jats:italic>H19<\/jats:italic> expression in K562 cells. Collectively, <jats:italic>H19<\/jats:italic> overexpression, a frequent event in CML, was associated with higher <jats:italic>BCR\u2010ABL<\/jats:italic> transcript involving in disease progression. Moreover, <jats:italic>H19<\/jats:italic> DMR\/ICR hypomethylation in CML may be one of the mechanisms mediating <jats:italic>H19<\/jats:italic> overexpression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26119","type":"journal-article","created":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T10:41:13Z","timestamp":1501843273000},"page":"2444-2450","source":"Crossref","is-referenced-by-count":23,"title":["Hypomethylation\u2010mediated <i>H19<\/i> overexpression increases the risk of disease evolution through the association with <i>BCR\u2010ABL<\/i> transcript in chronic myeloid leukemia"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1476-926X","authenticated-orcid":false,"given":"Jing\u2010dong","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Hematology Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"},{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Lin","sequence":"additional","affiliation":[{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"},{"name":"Laboratory Center Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting\u2010juan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"},{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010chun","family":"Ma","sequence":"additional","affiliation":[{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"},{"name":"Laboratory Center Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi\u2010xi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"},{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010mei","family":"Wen","sequence":"additional","affiliation":[{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"},{"name":"Laboratory Center Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Guo","sequence":"additional","affiliation":[{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"},{"name":"Laboratory Center Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010jun","family":"Xu","sequence":"additional","affiliation":[{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic of China"},{"name":"Laboratory Center Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao\u2010qun","family":"Deng","sequence":"additional","affiliation":[{"name":"The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City Zhenjiang Jiangsu People's Republic 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