{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T03:51:26Z","timestamp":1789271486862,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T00:00:00Z","timestamp":1504742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31171135"],"award-info":[{"award-number":["31171135"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472054"],"award-info":[{"award-number":["81472054"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1107828"],"award-info":[{"award-number":["1107828"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1027932"],"award-info":[{"award-number":["1027932"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Over\u2010production and activation of osteoclasts is a common feature of osteolytic conditions such as osteoporosis, tumor\u2010associated osteolysis, and inflammatory bone erosion. Cyanidin Chloride, a subclass of anthocyanin, displays antioxidant and anti\u2010carcinogenesis properties, but its role in osteoclastic bone resorption and osteoporosis is not well understood. In this study, we showed that Cyanidin Chloride inhibits osteoclast formation, hydroxyapatite resorption, and receptor activator of NF\u2010\u03baB ligand (RANKL)\u2010induced osteoclast marker gene expression; including <jats:italic>ctr<\/jats:italic>, <jats:italic>ctsk<\/jats:italic>, and <jats:italic>trap<\/jats:italic>. Further investigation revealed that Cyanidin Chloride inhibits RANKL\u2010induced NF\u2010\u03baB activation, suppresses the degradation of I\u03baB\u2010\u03b1 and attenuates the phosphorylation of extracellular signal\u2010regulated kinases (ERK). In addition, Cyanidin Chloride abrogated RANKL\u2010induced calcium oscillations, the activation of nuclear factor of activated T cells calcineurin\u2010dependent 1 (NFATc1), and the expression of c\u2010Fos. Further, we showed that Cyanidin Chloride protects against ovariectomy\u2010induced bone loss in vivo. Together our findings suggest that Cyanidin Chloride is capable of inhibiting osteoclast formation, hydroxyapatite resorption and RANKL\u2010induced signal pathways in vitro and OVX\u2010induced bone loss in vivo, and thus might have therapeutic potential for osteolytic diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26126","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T20:27:21Z","timestamp":1501792041000},"page":"2502-2512","source":"Crossref","is-referenced-by-count":57,"title":["Cyanidin Chloride inhibits ovariectomy\u2010induced osteoporosis by suppressing RANKL\u2010mediated osteoclastogenesis and associated signaling pathways"],"prefix":"10.1002","volume":"233","author":[{"given":"Jianwen","family":"Cheng","sequence":"first","affiliation":[{"name":"Research Center for Regenerative Medicine  and Guangxi Key Laboratory of Regenerative Medicine Guangxi Medical University Guangxi China"},{"name":"Department of Orthopaedic Surgery the First 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