{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:52Z","timestamp":1786977952196,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India","award":["BT\/RLF\/Re-entry\/36\/2013"],"award-info":[{"award-number":["BT\/RLF\/Re-entry\/36\/2013"]}]},{"name":"Department of Science and Technology (DST), Ministry of Science and Technology, Government of India","award":["YSS\/2014\/000125"],"award-info":[{"award-number":["YSS\/2014\/000125"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and an irreversible lung disorder characterized by the accumulation of fibroblasts and myofibroblasts in the extracellular matrix. The transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21)\u2010induced epithelial\u2010to\u2010mesenchymal transition (EMT) is thought to be one of the possible sources for a substantial increase in the number of fibroblasts\/myofibroblasts in IPF lungs. Tannic acid (TA), a natural dietary polyphenolic compound has been shown to possess diverse pharmacological effects. However, whether TA can inhibit TGF\u2010\u03b21\u2010mediated EMT in lung epithelial cells remains enigmatic. Both the human adenocarcinomic alveolar epithelial (A549) and normal bronchial epithelial (BEAS\u20102B) cells were treated with TGF\u2010\u03b21 with or without TA. Results showed that TA addition, markedly inhibited TGF\u2010\u03b21\u2010induced EMT as assessed by reduced expression of N\u2010cadherin, type\u20101\u2010collagen, fibronectin, and vimentin. Furthermore, TA inhibited TGF\u2010\u03b21\u2010induced cell proliferation through inducing cell cycle arrest at G0\/G1 phase. TGF\u2010\u03b21\u2010induced increase in the phosphorylation of Smad (Smad2 and 3), Akt as well as that of mitogen activated protein kinase (ERK1\/2, JNK1\/2, and p38) mediators was effectively inhibited by TA. On the other hand, TA reduced the TGF\u2010\u03b21\u2010induced increase in TGF\u2010\u03b2 receptors expression. Using molecular docking approach, FTIR, HPLC and Western blot analyses, we further identified the direct binding of TA to TGF\u2010\u03b21. Finally, we conclude that TA might directly interact with TGF\u2010\u03b21, thereby repressing TGF\u2010\u03b2 signaling and subsequent EMT process in lung epithelial cells. Further animal studies are needed to clarify its potential therapeutic benefit in pulmonary fibrosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26127","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T16:27:18Z","timestamp":1501777638000},"page":"2513-2525","source":"Crossref","is-referenced-by-count":71,"title":["Tannic acid attenuates TGF\u2010\u03b21\u2010induced epithelial\u2010to\u2010mesenchymal transition by effectively intervening TGF\u2010\u03b2 signaling in lung epithelial cells"],"prefix":"10.1002","volume":"233","author":[{"given":"Dhamotharan","family":"Pattarayan","sequence":"first","affiliation":[{"name":"Department of Biotechnology Anna University BIT\u2010Campus Tiruchirappalli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayyanar","family":"Sivanantham","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Anna University BIT\u2010Campus Tiruchirappalli 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