{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T01:40:56Z","timestamp":1780105256848,"version":"3.54.0"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T00:00:00Z","timestamp":1511827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>Radiocontrast media (RCM)\u2010induced acute kidney injury (CI\u2010AKI) is a major clinical problem whose pathophysiology is not well understood. Direct toxic effects on renal cells, possibly mediated by reactive oxygen species, have been postulated as contributing to CI\u2010AKI. We investigated the effect of quercetin on human renal proximal tubular (HK\u20102) cells treated with the radiocontrast medium (RCM) sodium diatrizoate. Quercetin is the most widely studied flavonoid, and the most abundant flavonol present in foods. It has been suggested to have many health benefits, including angioprotective properties and anti\u2010cancer effects. These beneficial effects have been attributed to its antioxidant properties and its ability to modulate cell signaling pathways. Incubation of HK\u20102 cells with 100\u2009\u03bcM quercetin caused a decrease in cell viability and pre\u2010treatment of HK\u20102 cells with 100\u2009\u03bcM quercetin followed by incubation with 75\u2009mgI\/ml sodium diatrizoate for 2\u2009hr caused a decrease in cell viability which was worse than in cells treated with diatrizoate alone. However, further incubation of the cells (for 22\u2009hr) after removal of the diatrizoate and quercetin caused a recovery in cell viability in those cells previously treated with quercetin\u2009+\u2009diatrizoate and quercetin alone. Analysis of signaling molecules by Western blotting showed that in RCM\u2010treated cells receiving initial pre\u2010treatment with quercetin, followed by its removal, an increase in phosphorylation of Akt (Ser473), pSTAT3 (Tyr705), and FoxO3a (Thr32) as well as an induction of Pim\u20101 and decrease in PARP1 cleavage were observed. Quercetin may alleviate the longer\u2010term toxic effects of RCM toxicity and its possible beneficial effects should be further investigated.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26213","type":"journal-article","created":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T20:00:48Z","timestamp":1508184048000},"page":"4116-4125","source":"Crossref","is-referenced-by-count":21,"title":["Quercetin protects against radiocontrast medium toxicity in human renal proximal tubular cells"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7974-3792","authenticated-orcid":false,"given":"Michele","family":"Andreucci","sequence":"first","affiliation":[{"name":"Department of Health Sciences (Nephrology Unit) \u201cMagna Graecia\u201d University Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Faga","sequence":"additional","affiliation":[{"name":"Department of Health Sciences (Nephrology Unit) \u201cMagna 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