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Our previous studies have confirmed that miR\u2010145\u20105p was abnormally elevated in CGN rats, but its mechanism remains unclear. Therefore, this study aimed to elucidate the mechanism of miR\u2010145\u20105p in regulation of renal mesangial cells proliferation and inflammatory responses. In vivo study, the cationic bovine serum albumin (C\u2010BSA)\u2010induced CGN rat model was established, and the content of miR\u2010145\u20105p in renal was examined by qRT\u2010PCR, meanwhile, we also determined the renal function and inflammatory infiltrate. In vitro, the cell proliferation rate, cell cycle and inflammatory changes of rat mesangial cells (RMCs) were measured. Our results suggested that miR\u2010145\u20105p extended the G0\u2010G1 phase, shortened S phase, inhibited cell proliferation and suppressed inflammatory responses in RMCs. Moreover, miR\u2010145\u20105p inhibited CXCL16 protein expression through binding the 3\u2032\u2010UTR of CXCL16, suppressed AKT\/GSK signaling pathway, and decreased expression of inflammation related mRNAs, such as IL\u20101\u03b1, IL\u20102, IL\u20106, and TNF\u2010\u03b1 mRNAs. Further, locking CXCL16 alleviated inflammatory reactions and down\u2010regulated AKT\/GSK pathway in RMCs. Above all, we concluded that miR\u2010145\u20105p inhibited proliferation and inflammatory responses of RMCs through regulation of AKT\/GSK pathway by targeting CXCL16.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26228","type":"journal-article","created":{"date-parts":[[2017,10,14]],"date-time":"2017-10-14T13:52:42Z","timestamp":1507989162000},"page":"3648-3659","source":"Crossref","is-referenced-by-count":33,"title":["MiR\u2010145\u20105p inhibits proliferation and inflammatory responses of RMC through regulating AKT\/GSK pathway by targeting CXCL16"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6053-6104","authenticated-orcid":false,"given":"Junbiao","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacy The Second Affiliated Hospital Guangzhou University of Chinese Medicine Guangzhou Guangdong P.R. 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