{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T01:45:38Z","timestamp":1789177538919,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,2]],"date-time":"2018-01-02T00:00:00Z","timestamp":1514851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["BSC\u20100101"],"award-info":[{"award-number":["BSC\u20100101"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>Mature and potentially motile spermatozoa stored in cauda epididymis in an inactive state for approximately 30 days; however, during ejaculation they regain motility. To understand the actual molecular mechanism of the sperm quiescence during caudal stay, a proteinaceous quiescence factor (QF) has been purified from caprine epididymal plasma to apparent homogeneity. In the present study complete purification, detailed characterization as well as mechanistic pathway of QF has been described. QF is purified to 215\u2010fold with 45% activity recovery. It is a 59\u2009kDa monomeric protein with isoelectric point 5.8 and optimally active at pH 7.5. Circular dichroism spectroscopy and atomic force microscopy study confirm its \u03b1\u2010helical secondary structure and globular tertiary conformation. QF is a thermo\u2010stable protein as higher temperature does not alter its helical structure. N\u2010terminal amino acid sequencing and MALDI analysis of QF did not find 100% similarity with any available protein of the database, proved its novelty. QF at 2\u2009\u03bcM dose inhibits sperm progressive forward motility within 10\u2009min. This motility inhibitory activity of QF is mediated by reducing NOS enzyme activity and subsequently decreasing the intracellular NO and cGMP concentration. It does not modulate intracellular Ca<jats:sup>++<\/jats:sup> and cAMP concentration. QF has no adverse effect on DNA integrity and morphology of spermatozoa. Motility inhibitory action of QF is reversible. Thus, the role of QF in maintaining energy saving quiescence state of mature cauda spermatozoa and its reactive nitrogen species reducing activity may lead to a new direction for storage of spermatozoa and idiopathic male infertility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26275","type":"journal-article","created":{"date-parts":[[2017,11,18]],"date-time":"2017-11-18T16:02:37Z","timestamp":1511020957000},"page":"4345-4359","source":"Crossref","is-referenced-by-count":8,"title":["A novel epididymal quiescence factor inhibits sperm motility by modulating NOS activity and intracellular NO\u2010cGMP pathway"],"prefix":"10.1002","volume":"233","author":[{"given":"Prasanta","family":"Ghosh","sequence":"first","affiliation":[{"name":"Cell Biology and Physiology Division, Sperm Biology Laboratory CSIR\u2010Indian Institute of Chemical Biology Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandipan","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Division, Sperm Biology Laboratory CSIR\u2010Indian Institute of Chemical Biology Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpita","family":"Bhoumik","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Division, Sperm Biology Laboratory CSIR\u2010Indian Institute of Chemical Biology Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0771-9187","authenticated-orcid":false,"given":"Sandhya R.","family":"Dungdung","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Division, Sperm Biology Laboratory CSIR\u2010Indian Institute of Chemical Biology Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod30.4.926"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb02113.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2007.00280.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0680275"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-014-0038-x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240350302"},{"key":"e_1_2_7_9_1","first-page":"787","article-title":"Adenine nucleotide changes at initiation of bull sperm motility","volume":"251","author":"Cascieri M.","year":"1976","journal-title":"Journal of Cellular Biochemistry"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmn055"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012039"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod28.4.788"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1984.tb38281.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.9.755"},{"key":"e_1_2_7_15_1","first-page":"247","article-title":"Sperm motility inhibitory protein in goat epididymal plasma","volume":"7","author":"Dungdung S. R.","year":"1995","journal-title":"Molecular Andrology"},{"issue":"3","key":"e_1_2_7_16_1","first-page":"413","article-title":"Isolation and identification of a novel motility\u2010inhibiting factor from goat cauda sperm plasma membrane","volume":"49","author":"Dungdung S. R.","year":"2003","journal-title":"Cellular and Molecular Biology"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400050101"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25958"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.suppl_4.20"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0460195"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)56766-1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1988.tb01069.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080310110"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1236"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2007.00282.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.05.007"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.12.020"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03424.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.11.873"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.07.001"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0151"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(95)00119-0"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(74)90852-3"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)44186-5"},{"key":"e_1_2_7_36_1","first-page":"350","article-title":"Proteinases in rat epididymis and testis","volume":"13","author":"Nag S.","year":"1975","journal-title":"Indian Journal of Experimental Biology"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.04.003"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-0679-9_21"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0830169"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2136654100"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012515400-0\/50027-0"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod55.4.813"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136174"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(89)90036-6"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.03.012"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20382"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.12.022"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/HUMREP\/11.3.561"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4660"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD05120"},{"key":"e_1_2_7_51_1","first-page":"199","article-title":"Sperm\u2010motility inhibiting factor in rat epididymis","volume":"242","author":"Turner T. T.","year":"1982","journal-title":"American Journal of Physiology"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/19.5.1095"},{"key":"e_1_2_7_53_1","first-page":"311","article-title":"On the epididymis and its function","volume":"16","author":"Turner T. T.","year":"1979","journal-title":"Investigative Urology"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1556\/AVet.2011.034"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod29.5.1241"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-55-17"},{"key":"e_1_2_7_57_1","first-page":"143","article-title":"Effect of papaya seed extract on microenvironment of cauda epididymis","volume":"3","author":"Verma R. J.","year":"2001","journal-title":"Asian Journal of Andrology"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)57743-7"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20853"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1978.tb00512.x"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26275","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26275","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.26275","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26275","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T12:25:28Z","timestamp":1693398328000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,2]]},"references-count":59,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1002\/jcp.26275"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26275","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,2]]}}}