{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T10:47:57Z","timestamp":1779187677214,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,2]],"date-time":"2018-01-02T00:00:00Z","timestamp":1514851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,1,2]],"date-time":"2018-01-02T00:00:00Z","timestamp":1514851200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Fund of China","doi-asserted-by":"crossref","award":["81503133"],"award-info":[{"award-number":["81503133"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds","award":["3010010004"],"award-info":[{"award-number":["3010010004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Aristolochic acid I (AA\u2010I), one of the main active components in\n                      <jats:italic>Aristolochaia<\/jats:italic>\n                      herbs, may induce aristolochic acid nephropathy (AAN). Renal interstitial fibrosis is one of the most typical features of AAN. To investigate the mechanism of Aristolochic acid I (AA\u2010I) \u2212induced renal epithelial\u2010mesenchymal transition (EMT) and determine the role of aquaporin\u20101 (AQP1) in this process, we established an AA\u2010I\u2010induced EMT model in human proximal tubular epithelial cells (HK\u20102 cells). Morphological examination, MTT assay, and Western blot analysis were performed. Aquaporin 1 (AQP1) and several EMT\u2010related proteins were detected, thereby suggesting the occurrence of AA\u2010I\u2010induced EMT. Two main pathways of transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) signaling, namely, Smad\u2010dependent and Smad\u2010independent signaling pathways, were also detected. The results showed that the TGF\u2010\u03b2 \/ Smad\u2010independent signaling pathways (\u03b2\u2010catenin, Ras\u2010Raf\u2010Erk1\/2 signaling pathways) were activated, and AQP1 expression was decreased during the AA\u2010I induced EMT on HK\u20102 cells. With the presence of TGF\u2010\u03b21 receptor inhibitor (LY364947) and Erk1\/2 inhibitor (PD98059), AQP1 expression was altered by PD98059, suggested that AQP1 could be adjusted by Erk1\/2 signaling. Moreover, the inhibitory effect of AA\u2010I on AQP1 was stronger than that of TGF\u2010\u03b21, suggested that AQP1 may be an important target on AAN clinical therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26310","type":"journal-article","created":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T10:59:32Z","timestamp":1512644372000},"page":"4919-4925","source":"Crossref","is-referenced-by-count":20,"title":["The potential role of aquaporin 1 on aristolochic acid I induced epithelial mesenchymal transition on HK\u20102 cells"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2931-798X","authenticated-orcid":false,"given":"Ji","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mincheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"},{"name":"Chia Tai Tianqing Pharmaceutical Group Co. Ltd. No. 699\u20108 Xuanwu District Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yimao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuehan","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy School of Preclinical Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1038\/nrd3878","article-title":"Targeting the TGF\u03b2 signaling pathway in disease","volume":"11","author":"Akhurst R. J.","year":"2012","journal-title":"Dressnature Reviews Drug Discovery"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000046647"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1016\/j.bbagen.2013.09.033","article-title":"Human aquaporins: Regulators of transcellular water flow","volume":"1840","author":"Day R. E.","year":"2013","journal-title":"Biochimica Et Biophysica Acta"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1681\/ASN.V132431","article-title":"Aristolochic acids induce chronic renal failure with interstitial fibrosis in salt\u2010depleted rats","volume":"13","author":"Debelle F. D.","year":"2002","journal-title":"Journal of the American Society of Nephrology"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005189"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2007.02.015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39104"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1159\/000446854","article-title":"Expression of renal aquaporins in aristolochic acid I and aristolactam I\u2010Induced nephrotoxicity","volume":"133","author":"Li J.","year":"2016","journal-title":"Nephron"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2010.03.012"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008121226"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3902"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.10.001"},{"key":"e_1_2_7_15_1","first-page":"204","article-title":"AQP1 is not only a water channel: It contributes to cell migration through lin7\/Beta\u2010Catenin","volume":"4","author":"Monzani E.","year":"2009","journal-title":"Cell Adhesion & Migration"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.ceb.2009.01.021","article-title":"Mechanism of TGF\u2010beta signaling to growth arrest, apoptosis, and epithelial\u2010mesenchymal transition","volume":"21","author":"Moustakas A.","year":"2009","journal-title":"Current Opinion in Cell Biology"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2009.231"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-007-0357-5"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2002.00333.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)90964-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92984-2"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4226"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2009.76"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005111187"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.12.128"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.13.8355"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26310","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26310","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.26310","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T10:57:06Z","timestamp":1773053826000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26310"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,2]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/jcp.26310"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26310","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,2]]}}}