{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:57:42Z","timestamp":1773079062524,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The isolation of good quality RNA from tissues is an essential prerequisite for gene expression analysis to study pathophysiological processes. This study evaluated the RNA isolated from human trabecular bone and defined a set of stable reference genes. After pulverization, RNA was extracted with a phenol\/chloroform method and then purified using silica columns. The A260\/280 ratio, A260\/230 ratio, RIN, and ribosomal ratio were measured to evaluate RNA quality and integrity. Moreover, the expression of six candidates was analyzed by qPCR and different algorithms were applied to assess reference gene stability. A good purity and quality of RNA was achieved according to A260\/280 and A260\/230 ratios, and RIN values.\n                      <jats:italic>TBP<\/jats:italic>\n                      ,\n                      <jats:italic>YWHAZ<\/jats:italic>\n                      , and\n                      <jats:italic>PGK1<\/jats:italic>\n                      were the most stable reference genes that should be used for gene expression analysis. In summary, the method proposed is suitable for gene expression evaluation in human bone and a set of reliable reference genes has been identified.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26319","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T08:44:20Z","timestamp":1512463460000},"page":"4401-4407","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of RNA from human trabecular bone and identification of stable reference genes"],"prefix":"10.1002","volume":"233","author":[{"given":"Simona","family":"Cepollaro","sequence":"first","affiliation":[{"name":"Laboratory of Preclinical and Surgical Studies Rizzoli Orthopedic Institute Bologna Italy"},{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5151-7390","authenticated-orcid":false,"given":"Elena","family":"Della Bella","sequence":"additional","affiliation":[{"name":"Laboratory of Preclinical and Surgical Studies Rizzoli Orthopedic Institute Bologna Italy"},{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dario","family":"de Biase","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Visani","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"Fini","sequence":"additional","affiliation":[{"name":"Laboratory of Preclinical and Surgical Studies Rizzoli Orthopedic Institute Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2010.01.010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.01755"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.03.011"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-12-5"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2006.07.032"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.12.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2001.6195"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364190"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.11.177"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.10.019"},{"key":"e_1_2_7_13_1","volume-title":"The principles of humane experimental technique","author":"Russell W. 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