{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:54:41Z","timestamp":1773078881259,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Scientific Research Foundation of Huashan Hospital","award":["2016QD10"],"award-info":[{"award-number":["2016QD10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Circulating tumor cells (CTCs) are an important topic of investigation for both basic and clinical cancer research. In this prospective study, we evaluated the clinical role of CTCs in ampullary cancer. We analyzed blood samples from 62 consecutively diagnosed patients with ampullary adenocarcinoma and 24 healthy controls for their CTC content. Combined data from immunostaining of CD45, 4\u2032,6\u2010diamidino\u20102\u2010phenylindole (DAPI), and fluorescence in situ hybridization with a chromosome 8 centromere (CEP8) probe were used to identify CTCs; cells that were CD45\u2010\/DAPI+\/CEP8&gt;2 were considered CTCs. The Cox proportional hazards model was used to assess the relationship between CTCs, clinical characteristics, and patient outcomes. We detected \u22652 CTCs\/3.2\u2009ml whole blood in 43 of 62 patients (69.4%), as well as \u22655 CTCs\/3.2\u2009ml in 16 of these patients (25.8%). A CTC cutoff value of 2 cells\/3.2\u2009ml achieved 69.4% sensitivity and 95.8% specificity as a diagnostic tool; CTCs were associated with tumor burden. CTC levels \u22653\/3.2\u2009ml (hazard ratio [HR]: 2.5, 95% confidence interval [CI]: (1.2\u20135.2),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.014) and \u22655\/3.2\u2009ml (HR: 3.5, 95% CI: 1.7\u20137.3,\n                      <jats:italic>p\u2009<\/jats:italic>\n                      &lt;\u20090.001) were both associated with shorter disease\u2010free survival. Moreover, \u22653 CTCs\/3.2\u2009ml (HR: 2.7, 95% CI: 1.2\u20136.3,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.019) and \u22655 CTCs\/3.2\u2009ml (HR: 3.8, 95% CI: 1.8\u20138.5,\n                      <jats:italic>p\u2009&lt;\u2009<\/jats:italic>\n                      0.001) were predictive of shorter overall survival. CTC assessment may help identify patients with ampullary cancer who are at high risk of an unfavorable outcome.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26353","type":"journal-article","created":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T09:13:04Z","timestamp":1512637984000},"page":"5014-5022","source":"Crossref","is-referenced-by-count":8,"title":["Prognostic impact of circulating tumor cells in patients with ampullary cancer"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1386-4612","authenticated-orcid":false,"given":"Bo","family":"Sun","sequence":"first","affiliation":[{"name":"Department of General Surgery  Huashan Hospital  Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengnan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of General Surgery  Huashan Hospital  Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingdang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,4]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.21374"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0378"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000230"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.1.38"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-014-0240-0"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.15.8923"},{"issue":"3","key":"e_1_2_13_8_1","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1053\/j.seminoncol.2006.03.016","article-title":"Circulating tumor cells, disease progression, and survival in metastatic breast cancer","volume":"33","author":"Cristofanilli M.","year":"2006","journal-title":"Seminars in Oncology"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2015.208"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.141"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-010-0985-4"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2008.10.026"},{"issue":"7","key":"e_1_2_13_13_1","first-page":"2073","article-title":"Cytogenetic evidence that circulating epithelial cells in patients with carcinoma are malignant","volume":"8","author":"Fehm T.","year":"2002","journal-title":"Clinical Cancer Research"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-016-0340-0"},{"issue":"1","key":"e_1_2_13_15_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.5858\/133.1.67","article-title":"Carcinomas of the pancreas, gallbladder, extrahepatic bile ducts, and ampulla of vater share a field for carcinogenesis: A population\u2010based study","volume":"133","author":"Henson D. 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