{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T21:47:00Z","timestamp":1776980820019,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T00:00:00Z","timestamp":1520380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370720"],"award-info":[{"award-number":["81370720"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>Single nucleotide polymorphisms (SNPs) in miRNAs are associated with the risk to development of certain human diseases and affect the regulatory capacity of miRNAs. However, the relationship between miRNAs polymorphisms and recurrent pregnancy loss (RPL) is still largely unknown. Our study found that one SNP rs56103835 T&gt;C in <jats:italic>miR\u2010323b<\/jats:italic> coding region was associated with the increase risk of human unexplained RPL (URPL), but no differences were found in another SNP rs75330474 C&gt;T. However, in two\u2010locus haplotype analysis, T\u2010C haplotype was associated with an increased risk of URPL. The level of mature <jats:italic>miR\u2010323b<\/jats:italic> was obviously up\u2010regulated in cells transfected with T\u2010C haplotype. T\u2010C haplotype inhibited HTR\u20108\/SVneo cells proliferation and migration and promoted cells apoptosis. Further experiments identified that paired\u2010box 8 (<jats:italic>Pax8<\/jats:italic>) was a functionally relevant target of <jats:italic>miR\u2010323b<\/jats:italic>, and its expression was reversely regulated by <jats:italic>miR\u2010323b<\/jats:italic>. Besides, the expressions of <jats:italic>Pax8<\/jats:italic> in villous chorionic tissues from URPL patients were lower than controls, contrary to the high expression of <jats:italic>miR\u2010323<\/jats:italic>. More importantly, dual\u2010luciferase assay indicated T\u2010C haplotype, increasing <jats:italic>miR\u2010323b<\/jats:italic> expression, could down\u2010regulated <jats:italic>Pax8<\/jats:italic> expression. Collectively, our data suggest that T\u2010C haplotype in pre\u2010miR\u2010323b may aggravate the risk of developing URPL and influence the level of mature <jats:italic>miR\u2010323b<\/jats:italic> and its target gene <jats:italic>Pax8<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.26415","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T08:40:14Z","timestamp":1514104814000},"page":"6001-6017","source":"Crossref","is-referenced-by-count":17,"title":["Haplotype\u2010based association of two SNPs in <i>miR\u2010323b<\/i> with unexplained recurrent spontaneous abortion in a Chinese Han population"],"prefix":"10.1002","volume":"233","author":[{"given":"Xue\u2010Qin","family":"Wang","sequence":"first","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"},{"name":"Graduate School Peking Union Medical College Beijing China"},{"name":"Department of Reproductive Medicine, Qingdao Municipal Hospital, School of Medicine Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"},{"name":"Graduate School Peking Union Medical College Beijing China"},{"name":"Department of pharmacy Xiamen Medical College Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Su","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"},{"name":"Graduate School Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"},{"name":"Graduate School Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haining","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Qingdao Municipal Hospital, School of Medicine Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Ma","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"},{"name":"Graduate School Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4872-5639","authenticated-orcid":false,"given":"Hongfei","family":"Xia","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Center of National Research Institute for Family Planning Beijing China"},{"name":"Graduate School Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki567"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2007.05.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2015.02.002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7754"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M041814"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.8.5.16034"},{"issue":"7","key":"e_1_2_8_9_1","first-page":"2600","article-title":"Genetic variants of miRNA sequences and non\u2010small cell lung cancer survival","volume":"118","author":"Hu Z.","year":"2008","journal-title":"Journal of Clinical Investigation"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2012.01577.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0000000000000245"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802682105"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h3080"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.04.040"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/8.5.463"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.06.102"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","unstructured":"Mendell J. 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