{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:46:10Z","timestamp":1787895970041,"version":"build-2784847793"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004391","name":"China Medical University Beigang Hospital","doi-asserted-by":"crossref","award":["CMUBH R105-006"],"award-info":[{"award-number":["CMUBH R105-006"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Aggrecan is a high molecular weight proteoglycan that plays a critical role in cartilage structure and the function of joints, providing intervertebral disc and cartilage with the ability to resist compressive loads. Aggrecan degradation in articular cartilage is a significant event in early\u2010stage osteoarthritis (OA). Currently, no effective treatment exists for OA other than pain relief. Dextrose (\n                      <jats:italic>D<\/jats:italic>\n                      \u2010glucose) prolotherapy has shown promising activity in the treatment of different musculoskeletal disorders, including OA. However, little is known about the molecular mechanism of the glucose effect in OA and on the regulation of chondrogenesis. We show for the first time that glucose upregulates aggrecan expression and subsequent chondrogenesis in ATDC5 cells. Moreover, we found that glucose\u2010induced aggrecan expression is mediated through the protein kinase C\u03b1 (PKC\u03b1)\u2010 and p38\u2010dependent pathway. As demonstrated by microRNA (miR) and luciferase analyses, the glucose\u2010induced PKC\u03b1\/p38 signaling axis is responsible for downregulating miR141\u20103p which targets to the 3\u2032untranslated region of aggrecan. In summary, we show that glucose enhances chondrogenesis by upregulating aggrecan expression via the PKC\u03b1\u2010p38\u2010miR141\u20103p signaling pathway. This result provides new insights into the mechanism of glucose on chondrogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26451","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T11:48:49Z","timestamp":1515584929000},"page":"6878-6887","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Glucose enhances aggrecan expression in chondrocytes via the PKC\u03b1\/p38\u2010miR141\u20103p signaling pathway"],"prefix":"10.1002","volume":"233","author":[{"given":"Tsung\u2010Ju","family":"Wu","sequence":"first","affiliation":[{"name":"Graduate Institute of Basic Medical Science China Medical University Taichung Taiwan"},{"name":"Department of Physical Medicine and Rehabilitation Changhua Christian Hospital Changhua Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chin","family":"Fong","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery China Medical University Beigang Hospital Yun\u2010Lin County Taiwan"},{"name":"Department of Sports Medicine College of Health Care China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih\u2010Yang","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Basic Medical Science China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3405-8863","authenticated-orcid":false,"given":"Yuan\u2010Li","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology College of Medical and Health Science Asia University Taichung Taiwan"},{"name":"Department of Medical Research China Medical University Hospital Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih\u2010Hsin","family":"Tang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Basic Medical Science China Medical University Taichung Taiwan"},{"name":"Department of Biotechnology College of Medical and Health Science Asia University Taichung Taiwan"},{"name":"Department of Pharmacology School of Medicine China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573399812666151123104352"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.5792\/ksrr.2016.28.3.219"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3283103d12"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmr.2015.08.412"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214100110"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-017-0390-6"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2016.10.021"},{"issue":"2","key":"e_1_2_8_9_1","first-page":"120","article-title":"Current concepts in osteoarthritis","volume":"21","author":"de Rezende M. 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