{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:58:39Z","timestamp":1785376719115,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T00:00:00Z","timestamp":1524614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T00:00:00Z","timestamp":1524614400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"The National Science and Technology Major Project","award":["2012ZX10005-005"],"award-info":[{"award-number":["2012ZX10005-005"]}]},{"name":"The National Science and Technology Major Project","award":["2014ZX10005001"],"award-info":[{"award-number":["2014ZX10005001"]}]},{"name":"Schistosomiasis Control Project in Hubei Province","award":["XF2012-17"],"award-info":[{"award-number":["XF2012-17"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The study aimed to investigate the impact of miR\u2010182 and\n                      <jats:italic>FOXO1<\/jats:italic>\n                      on\n                      <jats:italic>S. japonica<\/jats:italic>\n                      \u2010induced hepatic fibrosis. Microarray analysis was performed to screen out differential expressed miRNAs and mRNAs. Rat hepatic fibrosis model and human hepatocellular cell line LX\u20102 were used to study the effect of miR\u2010182 and\n                      <jats:italic>FOXO1<\/jats:italic>\n                      . qRT\u2010PCR and Western blot were used to detect the expression of miR\u2010182,\n                      <jats:italic>FOXO1<\/jats:italic>\n                      or other fibrosis markers. The targeting relationship between\n                      <jats:italic>FOXO1<\/jats:italic>\n                      and miR\u2010182 was verified by luciferase reporter assay. Immunohistochemistry or immunofluorescence staining was conducted to detect\n                      <jats:italic>FOXO1<\/jats:italic>\n                      or \u03b1\u2010SMA in rat hepatic tissues. Cell viability and apoptosis were detected by MTT assay and flow cytometry. The expression of\n                      <jats:italic>PI3K<\/jats:italic>\n                      \/\n                      <jats:italic>AKT<\/jats:italic>\n                      pathway\u2010related proteins was detected by Western blot. miR\u2010182 was highly expressed in liver fibrosis samples, and\n                      <jats:italic>FOXO1<\/jats:italic>\n                      expression was negatively correlated with miR\u2010182 expression. After transfection of miR\u2010182,\n                      <jats:italic>FOXO1<\/jats:italic>\n                      expression was down\u2010regulated, with the results of LX\u20102 cells proliferation inhibition and apoptosis induction, as well as the aggravation of rat hepatic fibrosis. The expression of\n                      <jats:italic>p\u2010AKT\/AKT<\/jats:italic>\n                      and\n                      <jats:italic>p\u2010S6\/S6<\/jats:italic>\n                      was increased, meaning that the\n                      <jats:italic>PI3K<\/jats:italic>\n                      \/\n                      <jats:italic>AKT<\/jats:italic>\n                      signal pathway was activated. The results were reversed when treated with Wortmannin (\n                      <jats:italic>PI3K<\/jats:italic>\n                      inhibitor). After transfection of miR\u2010182 inhibitor,\n                      <jats:italic>FOXO1<\/jats:italic>\n                      expression was up\u2010regulated, LX\u20102 cell proliferation was inhibited, and apoptosis rate was increased. High\u2010expressed miR\u2010182 and low\u2010expressed\n                      <jats:italic>FOXO1<\/jats:italic>\n                      promoted proliferation and inhibiting apoptosis on liver fibrosis cells, stimulating the development of\n                      <jats:italic>S. japonica<\/jats:italic>\n                      \u2010induced hepatic fibrosis through feeding back to PI3K\/AKT signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26469","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T09:55:42Z","timestamp":1515664542000},"page":"6693-6704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Effect of miR\u2010182 on hepatic fibrosis induced by\n                    <i>Schistosomiasis japonica<\/i>\n                    by targeting\n                    <i>FOXO1<\/i>\n                    through\n                    <i>PI3K<\/i>\n                    \/\n                    <i>AKT<\/i>\n                    signaling pathway"],"prefix":"10.1002","volume":"233","author":[{"given":"Yu","family":"Huang","sequence":"first","affiliation":[{"name":"Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"},{"name":"Department of Nephrology The First People's Hospital of Yichang, The People's Hospital of China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxue","family":"Fan","sequence":"additional","affiliation":[{"name":"Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"},{"name":"Department and Institute of Infectious Disease Liaocheng People's Hospital  Liaocheng Shandong 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