{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:56:57Z","timestamp":1787068617458,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T00:00:00Z","timestamp":1520380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT\u20101625"],"award-info":[{"award-number":["PICT\u20101625"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP\u2010413"],"award-info":[{"award-number":["PIP\u2010413"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP\u2010502"],"award-info":[{"award-number":["PIP\u2010502"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>It is known that bradykinin (BK) B2 receptor (B2R) is expressed in the collecting duct (CD) cells of the newborn rat kidney, but little is known about its role during early postnatal life. Therefore, we hypothesize that BK could participate in the mechanisms that mediate CD formation during the postnatal renal development. Performing primary cultures, combined with biochemical, immunocytochemical, and time\u2010lapse analysis, we studied the role of BK in CD cell behavior isolated from renal papilla of neonatal rats. A reverse relationship was observed between B2R expression and the degree of CD epithelial cell sheet maturation. BK stimulation induced CD cell association upon B2R activation. The lack of B2R expression in cells showing mature adherens junctions suggested that BK is mostly involved in early adhesive events, thus favoring the initial formation of CD during development. Time\u2010lapse analysis revealed that BK induced a high protrusive activity of CD cells, denoted by ruffle formation and lamellipodia extension. PI3K was involved in the BK\u2010induced CD cell\u2010cell association and the acquisition of the migratory phenotype since, when inhibited, membrane ruffles, and filopodia between cells diminished. Results indicate that the actions of BK mediated by PI3K activation were due to the downstream Akt and Rac pathways. This study, performed with CD cells that were not genetically manipulated, provides new experimental evidence supporting a novel role of BK in rat renal CD organization. As B2R blockade results in abnormal tubular differentiation, our results contribute to better understanding the etiology of human congenital renal malformation and diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26472","type":"journal-article","created":{"date-parts":[[2018,1,13]],"date-time":"2018-01-13T08:47:34Z","timestamp":1515833254000},"page":"6173-6195","source":"Crossref","is-referenced-by-count":4,"title":["Bradykinin mediates the association of collecting duct cells to form migratory colonies, through B2 receptor activation"],"prefix":"10.1002","volume":"233","author":[{"given":"Edith d. V.","family":"Guaytima","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Ciencias de la Salud Humana (IICSHUM) Universidad Nacional de La Rioja La Rioja Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamila R.","family":"Brand\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Ciencias de la Salud Humana (IICSHUM) Universidad Nacional de La Rioja La Rioja Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s O.","family":"Favale","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica y F\u00edsico\u2010Qu\u00edmica Biol\u00f3gica (IQUIFIB)\u2010CONICET Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"C\u00e1tedra de Biolog\u00eda Celular y Molecular Departamento de Ciencias Biol\u00f3gicas Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno J.","family":"Santacreu","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica y F\u00edsico\u2010Qu\u00edmica Biol\u00f3gica (IQUIFIB)\u2010CONICET Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"},{"name":"C\u00e1tedra de Biolog\u00eda Celular y Molecular Departamento de Ciencias Biol\u00f3gicas Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma B.","family":"Sterin\u2010Speziale","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica y F\u00edsico\u2010Qu\u00edmica Biol\u00f3gica (IQUIFIB)\u2010CONICET Facultad de Farmacia y Bioqu\u00edmica Universidad de Buenos Aires Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2575-0505","authenticated-orcid":false,"given":"Mar\u00eda G.","family":"M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Ciencias de la Salud Humana (IICSHUM) Universidad Nacional de La Rioja La Rioja Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.29.1.483"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(81)90262-2"},{"key":"e_1_2_6_4_1","first-page":"803","volume-title":"The kidney: Physiology and pathology","author":"Aperia A.","year":"1992"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3181b4f8c2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109663200"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.296.5573.1655"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06953"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)84716-6"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19971101)39:3<205::AID-JEMT1>3.0.CO;2-J"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.04.051"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.22.010305.104340"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.034876"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.88.3.275"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.108506"},{"key":"e_1_2_6_16_1","first-page":"F705","article-title":"Spatial and temporal expression of kallikrein and its mRNA during nephron maturation","volume":"262","author":"El\u2010Dahr S. 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