{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:24:27Z","timestamp":1790713467140,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T00:00:00Z","timestamp":1522368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T00:00:00Z","timestamp":1522368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL123093"],"award-info":[{"award-number":["HL123093"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL58672"],"award-info":[{"award-number":["HL58672"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL74854"],"award-info":[{"award-number":["HL74854"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL91799"],"award-info":[{"award-number":["HL91799"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Bcl2\u2010associated athanogene 3 (BAG3) is a 575 amino acid protein that is found predominantly in the heart, skeletal muscle, and many cancers. Deletions and truncations in BAG3 that result in haplo\u2010insufficiency have been associated with the development of dilated cardiomyopathy. To study the cellular and molecular events attributable to BAG3 haplo\u2010insufficiency we generated a mouse in which one allele of BAG3 was flanked by loxP recombination sites (BAG3\n                      <jats:sup>fl\/+<\/jats:sup>\n                      ). Mice were crossed with \u03b1\u2010MHC\u2010Cre mice in order to generate mice with cardiac\u2010specific haplo\u2010insufficiency (cBAG3\n                      <jats:sup>+\/\u2212)<\/jats:sup>\n                      and underwent bi\u2013weekly echocardiography to assess their cardiac phenotype. By 10 weeks of age, cBAG3\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                      mice demonstrated increased heart size and diminished left ventricular ejection fraction when compared with non\u2010transgenic littermates (Cre\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      BAG3\n                      <jats:sup>fl\/+<\/jats:sup>\n                      ). Contractility in adult myocytes isolated from cBAG3\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                      mice were similar to those isolated from control mice at baseline, but showed a significantly decreased response to adrenergic stimulation. Intracellular calcium ([Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ]\n                      <jats:sub>i<\/jats:sub>\n                      ) transient amplitudes in myocytes isolated from cBAG3\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                      mice were also similar to myocytes isolated from control mice at baseline but were significantly lower than myocytes from control mice in their response to isoproterenol. BAG3 haplo\u2010insufficiency was also associated with decreased autophagy flux and increased apoptosis. Taken together, these results suggest that mice in which BAG3 has been deleted from a single allele provide a model that mirrors the biology seen in patients with heart failure and BAG3 haplo\u2010insufficiency.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26482","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T12:03:54Z","timestamp":1515672234000},"page":"6319-6326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Haplo\u2010insufficiency of Bcl2\u2010associated athanogene 3 in mice results in progressive left ventricular dysfunction, \u03b2\u2010adrenergic insensitivity, and increased apoptosis"],"prefix":"10.1002","volume":"233","author":[{"given":"Valerie D.","family":"Myers","sequence":"first","affiliation":[{"name":"Department of Medicine Lewis Katz School of Medicine at Philadelphia Philadelphia 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