{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:12:27Z","timestamp":1787235147954,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T00:00:00Z","timestamp":1523318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T00:00:00Z","timestamp":1523318400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31270990"],"award-info":[{"award-number":["31270990"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11532004"],"award-info":[{"award-number":["11532004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Hepatic fibrosis progress accompanied by an unbalanced extracellular matrix (ECM) degradation and deposition leads to an increased tissue stiffness. Hepatocytes interplay with all intrahepatic cell populations inside the liver. However, how hepatocytes migration and cellular Young's modulus influenced by the substrate stiffness are not well understood. Here, we established a stiffness\u2010controllable in vitro cell culture model by using a polyvinyl alcohol (PVA) hydrogel that mimicked the same physical stiffness as a fibrotic liver. Three levels of stiffness were used in our experiment that corresponded to the stiffness levels found in normal liver tissue (4.5\u2009kPa), the early (19\u2009kPa) and late stages (37\u2009kPa) of fibrotic liver tissues. Cytoskeleton of hepatocyte was influenced by substrate stiffness. Soft substrate promoted the cellular migration and directionality. The cellular Young's modulus firstly increased and then decreased with increasing substrate stiffness. Integrin\u2010\u03b21 and \u03b2\u2010catenin expression on cytomembrane were up\u2010regulated and down\u2010regulated with the increase of substrate stiffness, respectively. Our data not only suggested that hepatocytes were sensitive to substrate stiffness, but also suggested that there may be a potential relationship among substrate stiffness, cellular Young's modulus and the dynamic balance of integrin\u2010\u03b21 and \u03b2\u2010catenin pathways. These results may provide us a new insight in mechanism investigation of mechano\u2010dependent diseases, especially like fibrosis related diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26491","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T10:15:18Z","timestamp":1516270518000},"page":"6996-7006","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Effect of substrate stiffness on hepatocyte migration and cellular Young's modulus"],"prefix":"10.1002","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9599-6899","authenticated-orcid":false,"given":"Tingting","family":"Xia","sequence":"first","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College Chongqing University Chongqing 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Biorheological Science and Technology, Ministry of Education, Bioengineering College Chongqing University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasinta N.","family":"Waju","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College Chongqing University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohanad K.","family":"Al\u2010ani","sequence":"additional","affiliation":[{"name":"Department of microbiology College of Medicine Tikrit University Tikrit Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonggang","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College Chongqing University Chongqing 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