{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:09:40Z","timestamp":1791972580823,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T00:00:00Z","timestamp":1525824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T00:00:00Z","timestamp":1525824000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003503","name":"Nederlandse Brandwonden Stichting","doi-asserted-by":"publisher","award":["13.101"],"award-info":[{"award-number":["13.101"]}],"id":[{"id":"10.13039\/501100003503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Abnormal cutaneous wound healing can lead to formation of fibrotic hypertrophic scars. Although several clinical risk factors have been described, the cross\u2010talk between different cell types resulting in hypertrophic scar formation is still poorly understood. The aim of this in vitro study was to investigate whether endothelial cells (EC) may play a role in skin fibrosis, for example, hypertrophic scar formation after full\u2010thickness skin trauma. Using a collagen\/elastin matrix, we developed an in vitro fibrosis model to study the interaction between EC and dermal fibroblasts or adipose tissue\u2010derived mesenchymal stromal cells (ASC). Tissue equivalents containing dermal fibroblasts and EC displayed a normal phenotype. In contrast, tissue equivalents containing ASC and EC displayed a fibrotic phenotype indicated by contraction of the matrix, higher gene expression of ACTA2, COL1A, COL3A, and less secretion of follistatin. The contraction was in part mediated via the TGF\u2010\u03b2 pathway, as both inhibition of the ALK4\/5\/7 receptors and the addition of recombinant follistatin resulted in decreased matrix contraction (75\u2009\u00b1\u200911% and 24\u2009\u00b1\u20098%, respectively). In conclusion, our study shows that EC may play a critical role in fibrotic events, as seen in hypertrophic scars, by stimulating ASC\u2010mediated matrix contraction via regulation of fibrosis\u2010related proteins.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26494","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T10:15:39Z","timestamp":1516270539000},"page":"6714-6722","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Endothelial cells enhance adipose mesenchymal stromal cell\u2010mediated matrix contraction via ALK receptors and reduced follistatin: Potential role of endothelial cells in skin fibrosis"],"prefix":"10.1002","volume":"233","author":[{"given":"Hanneke N.","family":"Monsuur","sequence":"first","affiliation":[{"name":"Department of Dermatology, VU University Medical Center Amsterdam Movement Sciences  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenie J.","family":"van den Broek","sequence":"additional","affiliation":[{"name":"Department of Dermatology, VU University Medical Center Amsterdam Movement Sciences  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Koolwijk","sequence":"additional","affiliation":[{"name":"Department of Physiology, Amsterdam Cardiovascular Sciences VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank B.","family":"Niessen","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Hand Surgery VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3446-6138","authenticated-orcid":false,"given":"Susan","family":"Gibbs","sequence":"additional","affiliation":[{"name":"Department of Dermatology, VU University Medical Center Amsterdam Movement Sciences  Amsterdam The Netherlands"},{"name":"Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam Amsterdam Movement Sciences  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1078\/0344-0338-00447"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.01.046"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62047-0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2008.00410.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2005.31612"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2008.07.016"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12380"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.00847.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0586"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4MR0316-102R"},{"issue":"3","key":"e_1_2_8_12_1","first-page":"377","article-title":"Angiogenesis and liver fibrosis","volume":"7","author":"Elpek G. 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