{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:11:54Z","timestamp":1787497914142,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Uterine leiomyomas (fibroids or myomas) are the most common benign tumors of premenopausal women and new medical treatments are needed. This study aimed to determine the effects of omega\u20103 fatty acids on the lipid profile, membrane architecture and gene expression patterns of extracellular matrix components (collagen1A1, fibronectin, versican, or activin A), mechanical signaling (integrin \u03b21, FAK, and AKAP13), sterol regulatory molecules (ABCG1, ABCA1, CAV1, and SREBF2), and mitochondrial enzyme (CYP11A1) in myometrial and leiomyoma cells. Myometrial tissues had a higher amount of arachidonic acid than leiomyoma tissues while leiomyoma tissues had a higher level of linoleic acid than myometrial tissues. Treatment of primary myometrial and leiomyoma cells with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) reduced the monounsaturated fatty acid (MUFA) content and increased the polyunsaturated fatty acid (PUFA) content in both cell types. Myometrial and leiomyoma cell membranes were in the liquid\u2010crystalline phase, but EPA\u2010 and DHA\u2010treated cells had decreased membrane fluidity. While we found no changes in the mRNA expression of ECM components, EPA and DHA treatment reduced levels of ABCG1, ABCA1, and AKAP13 in both cell types. EPA and DHA also reduced FAK and CYP11A1 expression in myometrial cells. The ability of omega\u20103 fatty acids to remodel membrane architecture and downregulate the expression of genes involved in mechanical signaling and lipid accumulation in leiomyoma cells offers to further investigate this compound as preventive and\/or therapeutic option.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26537","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T13:21:06Z","timestamp":1521984066000},"page":"7143-7156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Omega\u20103 fatty acids modulate the lipid profile, membrane architecture, and gene expression of leiomyoma cells"],"prefix":"10.1002","volume":"233","author":[{"given":"Md Soriful","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine Universit\u00e0 Politecnica delle Marche Ancona Italy"},{"name":"Biotechnology and Microbiology Laboratory Department of Botany University of Rajshahi Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Castellucci","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Sciences Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosamaria","family":"Fiorini","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Greco","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Gagliardi","sequence":"additional","affiliation":[{"name":"Eureka srl \u2212 Lab Division R&amp;D Department Chiaravalle (AN) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Zannotti","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano R.","family":"Giannubilo","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ciavattini","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natale G.","family":"Frega","sequence":"additional","affiliation":[{"name":"Department of Agricultural Food and Environmental Sciences Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Pacetti","sequence":"additional","affiliation":[{"name":"Department of Agricultural Food and Environmental Sciences Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4400-3786","authenticated-orcid":false,"given":"Pasquapina","family":"Ciarmela","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Universit\u00e0 Politecnica delle Marche Ancona Italy"},{"name":"Department of Information Engineering Universit\u00e0 Politecnica delle  Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9808215"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77050-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.03.055"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1209993"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)45789-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-001-0812-7"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2012-3647"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmr031"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/94.4.435"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02637052"},{"issue":"10","key":"e_1_2_6_12_1","first-page":"1503","article-title":"Uterine fibroid tumors: Diagnosis and treatment","volume":"75","author":"Evans P.","year":"2007","journal-title":"American Family Physician"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.5787"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1585401"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.02.002"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567205052772786"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.112.158295"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2013.12.021"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2336"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2016.1245649"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2623"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.03.007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114513002444"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.02.037"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ogc.2005.12.001"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(97)00534-6"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606718200"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2015.05.008"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2008.04.002"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(91)82041-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(93)90399-K"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.08.010"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2324-3"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20120268"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.11.057"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(99)00026-1"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2008.01.022"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2012.08644.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M300336-JLR200"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03622-9"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M700364-JLR200"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2012.04.010"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505368200"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1112063"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403506101"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.25.050304.092605"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060914"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719115584449"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26537","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26537","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.26537","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:18:03Z","timestamp":1787123883000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,25]]},"references-count":47,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1002\/jcp.26537"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26537","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,25]]},"assertion":[{"value":"2017-12-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}