{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:26Z","timestamp":1786978106938,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T00:00:00Z","timestamp":1529625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T00:00:00Z","timestamp":1529625600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010803","name":"Department of Biotechnology","doi-asserted-by":"crossref","award":["Sanction order number: BT\/SBIRI-1188\/SBIRI-23\/13"],"award-info":[{"award-number":["Sanction order number: BT\/SBIRI-1188\/SBIRI-23\/13"]}],"id":[{"id":"10.13039\/501100010803","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Osteoporosis is a major debilitating cause of fractures and decreases the quality of life in elderly patients. Bone homeostasis is maintained by bone forming osteoblasts and bone resorpting osteoclasts. Substantial evidences have shown that targeting osteoclasts using natural products is a promising strategy for the treatment of osteoporosis. In the current study, we investigated the osteoprotective effect of Abietic acid (AA) in in vitro and in vivo models of osteolysis. In vitro experiments demonstrated that, AA suppressed receptor activator of nuclear factor\u2010kappa B ligand (RANKL)\u2010induced osteoclastogenesis and F\u2010actin ring formation in a concentration dependent manner. Mechanistically, AA abrogated RANKL\u2010induced phosphorylation of IKK\u03b1\/\u03b2 (ser 176\/180), IkB\u03b1 (ser 32), and inhibited the nuclear translocation of NF\u2010\u03baB. We also found that, AA attenuated the RANKL\u2010induced phosphorylation of MAPKs and decreased the expression of osteoclast specific genes such as TRAP, DC\u2010STAMP, c\u2010Fos, and NFATc1. Consistent with in vitro results, in vivo Lipoploysaccharide (LPS)\u2010induced osteolysis model showed that AA inhibited the LPS\u2010induced serum surge in cytokines TNF\u2010\u03b1 and IL\u20106. \u03bc\u2010CT analysis showed that AA prevented the LPS\u2010induced osteolysis. Furthermore, histopathology and TRAP staining results suggested that AA decreased the number of osteoclasts in LPS\u2010injected mice. Taken together, we demonstrated that the osteoprotective action of AA is coupled with the inhibition of NF\u2010\u03baB and MAPK signaling and subsequent inhibition of NFATc1 and c\u2010Fos activities. Hence, AA may be considered as a promising drug candidate for the treatment of osteoporosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26575","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T07:22:41Z","timestamp":1529652161000},"page":"443-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Abietic acid attenuates RANKL induced osteoclastogenesis and inflammation associated osteolysis by inhibiting the NF\u2010KB and MAPK signaling"],"prefix":"10.1002","volume":"234","author":[{"given":"Dinesh","family":"Thummuri","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and Research\u2010Hyderabad Balanagar Hyderabad Andhra Pradesh 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