{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:51:36Z","timestamp":1760550696575,"version":"3.41.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,11]],"date-time":"2018-11-11T00:00:00Z","timestamp":1541894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liver injury is an important cause of serious liver disease. This study aims to explore the effects of miR\u2010217 targeting NAT2 on hepatocyte proliferation, apoptosis, and autophagy following carbon tetrachloride (CCL4)\u2010induced liver injury. Rat models of CCL4\u2010induced liver injury were established. Healthy Wistar rats were randomized into the normal, blank, negative control (NC), microRNA\u2010217 (miR\u2010217) mimic, miR\u2010217 inhibitor, small interfering RNA (siRNA)\u2010<jats:italic>N<\/jats:italic>\u2010acetyltransferase 2 (NAT2), and miR\u2010217 inhibitor\u2009+\u2009siRNA\u2010NAT2 groups. NAT2 activity was evaluated with reversed\u2010phase high\u2010performance liquid chromatographic method.\u00a0Immunohistochemistry was used to detect NAT2 protein positive rate. Reverse transcription quantitative polymerase chain reaction and western blot analysis were used to examine expressions of miR\u2010217, NAT2, Bcl\u20102, Bax, p35, LC3\u2010II, Becline\u20101, and the ratio of caspase\u20103\/cleaved caspase\u20103. Autophagy, proliferation, and cell cycle distribution were determined by electron microscope, CCK\u20108, and flow cytometry. NAT2 protein positive rate and miR\u2010217, NAT2, Bcl\u20102, and p35 expressions were higher and Bax, LC3\u2010II, and Becline\u20101 expressions and the ratio of caspase\u20103\/cleaved caspase\u20103 lower in the normal group than the other six groups. Compared with the blank and NC groups, in the miR\u2010217 mimic and siRNA\u2010NAT2 groups, Bax, LC3\u2010II, and Becline\u20101 expressions and the ratio of caspase\u20103\/cleaved caspase\u20103, and hepatocyte apoptosis and autophagy increased, while NAT2, Bcl\u20102, and p35 expressions and hepatocyte proliferation decreased; opposite results were observed in the miR\u2010217 inhibitor group. Collectively, miR\u2010217 targeting NAT2 inhibits proliferation and promotes apoptosis and autophagy of hepatocytes in CCL4\u2010induced liver injury.<\/jats:p>","DOI":"10.1002\/jcp.26748","type":"journal-article","created":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T06:05:32Z","timestamp":1542002732000},"page":"3410-3424","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effects of microRNA\u2010217 on proliferation, apoptosis, and autophagy of hepatocytes in rat models of CCL4\u2010induced liver injury by targeting NAT2"],"prefix":"10.1002","volume":"234","author":[{"given":"Cheng\u2010Liang","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Li","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Nan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Fei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8456-3852","authenticated-orcid":false,"given":"Hong","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology The Affiliated Cancer Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Heping Hospital of Changzhi Medical College Changzhi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,11]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.187101"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.3797\/scipharm.1006-02"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-012-1429-9"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2016.08.009"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020238"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080266"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/378484"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00368.x"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25761"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.356733"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-990"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.4238\/2014.November.11.11"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23696"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2009.04.007"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/1354750X.2014.944217"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2017.00065"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1208\/aapsj080106"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.4238\/2015.August.10.16"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.6289"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2016.02.003"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.03.002"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.06.010"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.11.008"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2010.02.006"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-014-1703-0"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2011.03.006"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/nep.12715"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2015.11.014"},{"key":"e_1_2_7_1_30_1","first-page":"1","article-title":"Expression of autophagy related genes mTOR, Becline\u20101, LC3, and p62 in the peripheral blood mononuclear cells of systemic lupus erythematosus","volume":"6","author":"Wu Z. Z.","year":"2017","journal-title":"American Journal of Clinical and Experimental Immunology"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.01.030"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.5249"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.14266"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13089808"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176395"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i22.2771"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26748","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26748","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.26748","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T10:27:32Z","timestamp":1747996052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,11]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/jcp.26748"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26748","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"type":"print","value":"0021-9541"},{"type":"electronic","value":"1097-4652"}],"subject":[],"published":{"date-parts":[[2018,11,11]]},"assertion":[{"value":"2017-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}