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However, the role of AP in attenuating the progression of OA is still unknown. We hypothesized that its therapeutic effect may be associated with its antioxidant potential. In this study, we investigated the therapeutic effect of AP on chondrocytes injured by H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and the association with the oxidation\u2010related signaling pathways through the detection of cell proliferation, cell viability, the expression of oxidative stress\u2010specific genes (\n                    <jats:italic>Sod1<\/jats:italic>\n                    ,\n                    <jats:italic>Cat<\/jats:italic>\n                    , and\n                    <jats:italic>malonaldehyde<\/jats:italic>\n                    [\n                    <jats:italic>Mda<\/jats:italic>\n                    ]) and proteins (superoxide dismutase [SOD], catalase [CAT]) after a culture period of 3 and 5 days, respectively. Further exploration of the expression of\n                    <jats:italic>nuclear factor erythroid 2\u2010related factor 2<\/jats:italic>\n                    (\n                    <jats:italic>Nrf2<\/jats:italic>\n                    )\u00a0messenger RNA and protein was also performed. The results showed that 0.625\u2009\u00b5g\/ml and 2.5\u2009\u00b5g\/ml of AP decreased oxidative stress injury of chondrocytes by increasing cell proliferation reduced by H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and antioxidant enzyme activity, including SOD and CAT. Inflammation factors, such as\n                    <jats:italic>matrix metallopeptidase 13 (Mmp13)<\/jats:italic>\n                    ,\n                    <jats:italic>tissue inhibitor of metalloproteinase 1 (Timp1)<\/jats:italic>\n                    , and\n                    <jats:italic>interleukin\u20106 (Il6)<\/jats:italic>\n                    , were downregulated in the H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    group with AP, demonstrating a decrease in the progression of OA. Pathway analyses identified that the kelch\u2010like ECH\u2010associated protein 1 (Keap1)\u2013Nrf2\u2013antioxidant response element (Are) pathway is an important mediator in AP therapy on H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced OA. This study indicates that AP exerts protection effects on oxidative stress via activation of the Keap1\u2013Nrf2\u2013Are pathway in chondrocytes injured by H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , which may be promising for the therapy of OA.\n                  <\/jats:p>","DOI":"10.1002\/jcp.26769","type":"journal-article","created":{"date-parts":[[2018,8,6]],"date-time":"2018-08-06T23:40:50Z","timestamp":1533598850000},"page":"561-571","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Andrographolide protects chondrocytes from oxidative stress injury by activation of the Keap1\u2013Nrf2\u2013Are signaling pathway"],"prefix":"10.1002","volume":"234","author":[{"given":"Bo","family":"Li","sequence":"first","affiliation":[{"name":"Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration The First Affiliated Hospital of Guangxi Medical University Nanning China"},{"name":"Guangxi Collaborative Innovation Center for Biomedicine The First Affiliated Hospital of Guangxi Medical University Nanning China"},{"name":"Department of Orthopaedics Trauma and Hand Surgery The First Affiliated Hospital of Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6902-1980","authenticated-orcid":false,"given":"Tongmeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration The First Affiliated Hospital of Guangxi Medical University Nanning China"},{"name":"Guangxi Collaborative Innovation Center for Biomedicine The First Affiliated Hospital of Guangxi Medical University Nanning China"},{"name":"Department of Orthopaedics Trauma and Hand Surgery The First Affiliated Hospital of Guangxi Medical University Nanning 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