{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:27:26Z","timestamp":1770751646422,"version":"3.50.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81660156"],"award-info":[{"award-number":["81660156"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760136"],"award-info":[{"award-number":["81760136"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003996","name":"Kunming Medical University","doi-asserted-by":"publisher","award":["2014FZ048"],"award-info":[{"award-number":["2014FZ048"]}],"id":[{"id":"10.13039\/501100003996","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003996","name":"Kunming Medical University","doi-asserted-by":"publisher","award":["2017FE468(\u2010181)"],"award-info":[{"award-number":["2017FE468(\u2010181)"]}],"id":[{"id":"10.13039\/501100003996","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003996","name":"Kunming Medical University","doi-asserted-by":"publisher","award":["2018"],"award-info":[{"award-number":["2018"]}],"id":[{"id":"10.13039\/501100003996","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human mesenchymal stem cells (hMSCs) are fibroblastoid multipotent adult stem cells with capacities of differentiation into osteoblasts and chondrocytes and show great potential in new bone formation and bone repair\u2010related clinical settings, such as osteoporosis. Long noncoding RNAs (lncRNAs) have been demonstrated to play important roles in various biological processes. Here, we report an antisense lncRNA SEMA3B\u2010AS1 regulating hMSCs osteogenesis. SEMA3B\u2010AS1 is proximal to a member of the semaphorin family Sema3b. Overexpression of SEMA3B\u2010AS1 using the lentivirus system markedly inhibits the proliferation of hMSCs and meanwhile reduces osteogenic differentiation. Using a comprehensive proteomic technique named isobaric tag for relative and absolute quantitation, we found that SEMA3B\u2010AS1 significantly alters the process of osteogenesis through downregulating the expression of proteins involved in actin cytoskeleton, focal adhesion, and extracellular matrix\u2013receptor interaction, while increasing the expression of proteins in the spliceosome. Collectively, we find that SEMA3B\u2010AS1 is a target for controlling osteogenesis of hMSCs.<\/jats:p>","DOI":"10.1002\/jcp.26776","type":"journal-article","created":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T11:14:23Z","timestamp":1539515663000},"page":"2491-2499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["SEMA3B\u2010AS1\u2010inhibited osteogenic differentiation of human mesenchymal stem cells revealed by quantitative proteomics analysis"],"prefix":"10.1002","volume":"234","author":[{"given":"Chen","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery The People's Hospital of Yuxi City The 6th Affiliated Hospital of Kunming Medical University Yuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Health Screening Center The People's Hospital of Yuxi City The 6th Affiliated Hospital of Kunming Medical University Yuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yugang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Affiliated Hospital of Hebei University of Engineering Handan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The People's Hospital of Yuxi City The 6th Affiliated Hospital of Kunming Medical University Yuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoning","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The People's Hospital of Yuxi City The 6th Affiliated Hospital of Kunming Medical University Yuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Medical Research Center Wuhan GeneCreate Biological Engineering Co., Ltd. Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yatang","family":"Chen","sequence":"additional","affiliation":[{"name":"Medical Research Center Wuhan GeneCreate Biological Engineering Co., Ltd. Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Kunming General Hospital PLA Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaowei","family":"Teng","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery The People's Hospital of Yuxi City The 6th Affiliated Hospital of Kunming Medical University Yuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,14]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt318"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr700747q"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00164"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/619832"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106750\u201013\u201098"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447839"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741\u20107015\u20109\u201066"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3606"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.17329"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863\u2010017\u20100569\u20104"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11000"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000478751"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2225"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.10.007"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2439"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10697"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2116"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010017\u20103015\u2010z"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20121"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.18655"},{"key":"e_1_2_6_1_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014\u20101443"},{"key":"e_1_2_6_1_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEB.2012.0014"},{"key":"e_1_2_6_1_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2489"},{"key":"e_1_2_6_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20174"},{"key":"e_1_2_6_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2015.10"},{"key":"e_1_2_6_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.12.012"},{"key":"e_1_2_6_1_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T600050\u2010MCP200"},{"key":"e_1_2_6_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1416"},{"key":"e_1_2_6_1_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16023251"},{"key":"e_1_2_6_1_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.125"},{"key":"e_1_2_6_1_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169097"},{"key":"e_1_2_6_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4337"},{"key":"e_1_2_6_1_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010017\u20100485\u20106"},{"key":"e_1_2_6_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2322"},{"key":"e_1_2_6_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2016.07.036"},{"key":"e_1_2_6_1_38_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11538"},{"key":"e_1_2_6_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1989"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26776","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26776","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.26776","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T05:05:51Z","timestamp":1744261551000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,14]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/jcp.26776"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26776","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,14]]},"assertion":[{"value":"2018-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}