{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:28:49Z","timestamp":1790267329122,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300713"],"award-info":[{"award-number":["81300713"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["17ZR1425900"],"award-info":[{"award-number":["17ZR1425900"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inhibition of osteoclasts formation and bone resorption by estrogen is very important in the etiology of postmenopausal osteoporosis. The mechanisms of this process are still not fully understood. Recent studies implicated an important role of microRNAs in estrogen\u2010mediated responses in various cellular processes, including cell differentiation and proliferation. Thus, we hypothesized that these regulatory molecules might be implicated in the process of estrogen\u2010decreased osteoclasts formation and bone resorption. Western blot, quantitative real\u2010time polymerase chain reaction, tartrate\u2010resistant acid phosphatase staining, pit formation assay and luciferase assay were used to investigate the role of microRNAs in estrogen\u2010inhibited osteoclast differentiation and bone resorption. We found that estrogen could directly suppress receptor activator of nuclear factor B ligand\/macrophage colony\u2010stimulating factor\u2010induced differentiation of bone marrow\u2010derived macrophages into osteoclasts in the absence of stromal cell. MicroRNA\u201027a was significantly increased during the process of estrogen\u2010decreased osteoclast differentiation. Overexpressing of microRNA\u201027a remarkably enhanced the inhibitory effect of estrogen on osteoclast differentiation and bone resorption, whereas which were alleviated by microRNA\u201027a depletion. Mechanistic studies showed that microRNA\u201027a inhibited peroxisome proliferator\u2010activated receptor gamma (PPAR\u03b3) and adenomatous polyposis coli (APC) expression in osteoclasts through a microRNA\u201027a binding site within the 3\u2032\u2010untranslational region of PPAR\u03b3 and APC. PPAR\u03b3 and APC respectively contributed to microRNA\u201027a\u2010decreased osteoclast differentiation and bone resorption. Taken together, these results showed that microRNA\u201027a may play a significant role in the process of estrogen\u2010inhibited osteoclast differentiation and function.<\/jats:p>","DOI":"10.1002\/jcp.26788","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T02:54:03Z","timestamp":1536634443000},"page":"581-594","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Estrogen inhibits osteoclasts formation and bone resorption via microRNA\u201027a targeting PPAR\u03b3 and APC"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5143-3234","authenticated-orcid":false,"given":"Lei","family":"Guo","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory for Bone and Joint Diseases Shanghai Institute of Orthopaedics and Traumatology, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai 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