{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:13:06Z","timestamp":1775740386183,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recently, microRNAs (miRNAs) have been demonstrated to participate in many physiological and biological processes, especially by acting as circulating biomarkers or modulators in cell differentiation. Therefore, the aim of the current study was to clarify whether microRNA\u2010320a (miR\u2010320a) regulates the proliferation, migration, invasion, and apoptosis of trophoblasts and endothelial cells. In this study, miR\u2010320a mimics and inhibitors were transfected into HTR.8\/SVneo cells and human umbilical vein endothelial cells (HUVECs) using liposomes. Subsequently, the expression of miR\u2010320a and estrogen\u2010related receptor \u03b3 (ERR\u03b3) mRNA was detected by a reverse transcription quantitative polymerase chain reaction, whereas the protein expression of ERR\u03b3, vascular endothelial growth factor (VEGF), angiogenin 1 (Ang\u20101), human 3beta\u2010hydroxysteroid dehydrogenase type 1 (HSD3B1), and human chorionic gonadotropin (HCG) was detected by western blot analysis. Furthermore, the proliferation, invasion\/migration, and apoptosis of cells were analyzed by the cell counting kit\u20108 assay, transwell assay, and flow cytometry, respectively. The results showed that overexpression of miR\u2010320a decreased the optical density (OD) values and the proliferation rate of HTR.8\/SVneo cells and HUVECs, while inhibiting the expression of VEGF, Ang\u20101, HSD3B1, and HCG in these cells. Furthermore, miR\u2010320a reduced the ability of cell invasion and migration, while increasing the rate of cell apoptosis. After cotransfecting the cells with miR\u2010320a and ERR\u03b3 small (or short) interfering RNA (siRNA), the decreased ERR\u03b3 expression led to inhibited proliferation, migration, and invasion, but increased apoptosis of HTR.8\/SVneo cells and HUVECs. Our results further revealed that miR\u2010320a induced the apoptosis of trophoblasts and endothelial cells while inhibiting their proliferation, migration, and invasion by decreasing the expression of ERR\u03b3 and by indirectly suppressing the expression of VEGF, Ang\u20101, HSD3B1, and HCG.<\/jats:p>","DOI":"10.1002\/jcp.26842","type":"journal-article","created":{"date-parts":[[2018,9,15]],"date-time":"2018-09-15T22:08:42Z","timestamp":1537049322000},"page":"682-691","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Regulatory role of microRNA\u2010320a in the proliferation, migration, invasion, and apoptosis of trophoblasts and endothelial cells by targeting estrogen\u2010related receptor \u03b3"],"prefix":"10.1002","volume":"234","author":[{"given":"Rong\u2010Hua","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Linyi People\u2019s Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Meng","sequence":"additional","affiliation":[{"name":"Teaching and Research Department of Obstetrics and Gynecology Shandong Medical College Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Ping","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Linyi People\u2019s Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9813-6464","authenticated-orcid":false,"given":"Huai\u2010Sheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Linyi People\u2019s Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,14]]},"reference":[{"issue":"26442","key":"e_1_2_7_1_2_1","article-title":"Exercise\u2010like effects by estrogen\u2010related receptor\u2010gamma in muscle do not prevent insulin resistance in db\/db mice","volume":"6","author":"Badin P. 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