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The aim of the present study was to analyze the role of FGF\u20102 in the maintenance of sperm physiology using FGF\u20102 knockout (KO) mice. Our results showed that in wild\u2010type (WT) animals, FGF\u20102 is expressed in germ cells of the seminiferous epithelium, in epithelial cells of the epididymis, and in the flagellum and acrosomal region of epididymal sperm. In the FGF\u20102 KO mice, we found alterations in spermatogenesis kinetics, higher numbers of spermatids per testis, and enhanced daily sperm production compared with the WT males. No difference in the percentage of sperm motility was detected, but a significant increase in sperm concentration and in sperm head abnormalities was observed in FGF\u20102 KO animals. Sperm from KO mice depicted reduced phosphorylation on tyrosine residues (a phenomenon that was associated with sperm capacitation) and increased acrosomal loss after incubation under capacitating conditions. However, the FGF\u20102 KO males displayed no apparent fertility defects, since their mating with WT females showed no differences in the time to delivery, litter size, and pup weight in comparison with WT males. Overall, our findings suggest that FGF\u20102 exerts a role in mammalian spermatogenesis and that the lack of FGF\u20102 leads to dysregulated sperm production and altered sperm morphology and function. FGF\u20102\u2010deficient mice constitute a model for the study of the complex mechanisms underlying mammalian spermatogenesis.<\/jats:p>","DOI":"10.1002\/jcp.26876","type":"journal-article","created":{"date-parts":[[2018,7,28]],"date-time":"2018-07-28T05:28:29Z","timestamp":1532755709000},"page":"9640-9651","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Deficiency of fibroblast growth factor 2 (FGF\u20102) leads to abnormal spermatogenesis and altered sperm physiology"],"prefix":"10.1002","volume":"233","author":[{"given":"Luc\u00eda","family":"Saucedo","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME), National Research Council of Argentina (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Rumpel","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"Sobarzo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, National Research Council of Argentina (CONICET), University of Buenos Aires (UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Schreiner","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gudrun","family":"Brandes","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia","family":"Lustig","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas, National Research Council of Argentina (CONICET), University of Buenos Aires (UBA) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica Hebe","family":"Vazquez\u2010Levin","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME), National Research Council of Argentina (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Grothe","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-3758","authenticated-orcid":false,"given":"Clara","family":"Mar\u00edn\u2010Briggiler","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME), National Research Council of Argentina (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,27]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2016.03.002"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a015958"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.056770"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1640149"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD11318"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02704"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2007-0028"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4213"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2005.01.001"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2010-0142"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod13.5.513"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12398"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00381.2011"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24649"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.7.7.889"},{"key":"e_1_2_6_1_17_1","first-page":"47","article-title":"Testicular regulation of epididymal gene expression","volume":"3","author":"Hinton B. 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