{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:13:11Z","timestamp":1775740391746,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T00:00:00Z","timestamp":1535068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T00:00:00Z","timestamp":1535068800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300828"],"award-info":[{"award-number":["81300828"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      Osteoarthritis (OA) is a degenerative joint disease that leads to the destruction of joint function. The aim of this study is to investigate the effects of microRNA\u2010340\u20105p (miR\u2010340\u20105p) and its target gene,\n                      <jats:italic>FMOD<\/jats:italic>\n                      ,\u00a0on the proliferation and apoptosis of chondrocytes in mice with OA through the extracellular signal\u2010regulated kinase (ERK) signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials<\/jats:title>\n                    <jats:p>Twenty\u00a0healthy C57BL\/6J mice aged 15 months with a weight of 50\u2009\u00b1\u20092\u2009g were selected. Ten mice were treated using a unilateral knee anterior cruciate ligament transection as well as a medial meniscectomy to establish the OA model. Besides, another 10 mice were used as the control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A\u00a0reverse transcription quantitative polymerase chain reaction and Western blot analysis methods were used to examine the expressions of related genes in cells of each group. A\u00a03\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)2,5\u2010diphenyl tetrazolium bromide assay and flow cytometry were also conducted to evaluate the cell function after transfection had been completed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The expressions of fibromodulin (FMOD), type II collagen (Col II), B\u2010cell lymphoma\u20102 (Bcl\u20102), sex\u2010determining region of Y chromosome (SRY)\u2010related high\u2010mobility group\u2010box gene 9 (Sox9), and proliferating cell nuclear antigen (PCNA) were decreased, whereas the expressions of miR\u2010340\u20105p, runt\u2010related transcription factor\u20102 (Runx2), Bcl\u20102\u2010associated X protein (Bax), and ERK1\/2 were elevated in the OA mice. Downregulation of miR\u2010340\u20105p and upregulation of\n                      <jats:italic>FMOD<\/jats:italic>\n                      decreased the expressions of Runx2, Bax, and ERK1\/2, and cell apoptosis of chondrocytes, and increased the expressions of FMOD, Col II, Bcl\u20102, Sox9, and PCNA, and cell proliferation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study suggests that downregulation of miR\u2010340\u20105p plays a role in promoting cell proliferation and suppressing cell apoptosis of chondrocytes in OA mice through inhibition of the ERK signaling pathway via the\n                      <jats:italic>FMOD<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.26921","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T10:23:36Z","timestamp":1535106216000},"page":"927-939","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Downregulated microRNA\u2010340\u20105p promotes proliferation and inhibits apoptosis of chondrocytes in osteoarthritis mice through inhibiting the extracellular signal\u2010regulated kinase signaling pathway by negatively targeting the\n                    <i>FMOD<\/i>\n                    gene"],"prefix":"10.1002","volume":"234","author":[{"given":"Weikai","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihua","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weifeng","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengjing","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anmin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4741-4361","authenticated-orcid":false,"given":"Hui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,24]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1171104"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.12.110"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2011.12.012"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60243-2"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms15010560"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242606799"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6215-12-160"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13905"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12559"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.267"},{"key":"e_1_2_7_1_12_1","first-page":"441","article-title":"Research progress on MAPK signal pathway in the pathogenesis of osteoarthritis","volume":"27","author":"Gao S. 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