{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:10:54Z","timestamp":1777939854147,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,5]],"date-time":"2018-08-05T00:00:00Z","timestamp":1533427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,8,5]],"date-time":"2018-08-05T00:00:00Z","timestamp":1533427200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["431465\/2016\u20109"],"award-info":[{"award-number":["431465\/2016\u20109"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/16196\u20102"],"award-info":[{"award-number":["2017\/16196\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/05402\u20100"],"award-info":[{"award-number":["2017\/05402\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/04194\u20100"],"award-info":[{"award-number":["2015\/04194\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/15827\u20106"],"award-info":[{"award-number":["2016\/15827\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>\n                    The mechanisms of cancer involve changes in multiple biological pathways. Multitarget molecules, which are components of animal venoms, are therefore a potential strategy for treating tumors. The objective of this study was to screen the effects of\n                    <jats:italic>Phoneutria nigriventer<\/jats:italic>\n                    spider venom (PnV) on tumor cell lines. Cultured human glioma (NG97), glioblastoma (U\u2010251) and cervix adenocarcinoma (HeLa) cells, and nontumor mouse fibroblasts (L929) were treated with low (14\u2009\u00b5g\/ml) and high (280\u2009\u00b5g\/ml) concentrations of PnV, and analyzed through assays for cell viability (thiazolyl blue tetrazolium blue), proliferation (carboxyfluorescein succinimidyl ester), death (annexin V\/propidium iodide [Pi]), the cell cycle (Pi), and migration (wound healing and transwell assay). The venom decreased the viability of U\u2010251 cells, primarily by inducing cell death, and reduced the viability of NG97 cells, primarily by inhibiting the cell cycle. The migration of all the tumor cell lines was delayed when treated with venom. The venom significantly affected all the tumor cell lines studied, with no cytotoxic effect on normal cells (L929), although the nonglial tumor cell (HeLa) was less sensitive to PnV. The results of the current study suggest that PnV may be composed of peptides that are highly specific for the multiple targets involved in the hallmarks of cancer. Experiments are underway to identify these molecules.\n                  <\/jats:p>","DOI":"10.1002\/jcp.26935","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T02:58:31Z","timestamp":1533610711000},"page":"1398-1415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Venom of the\n                    <i>Phoneutria nigriventer<\/i>\n                    spider alters the cell cycle, viability, and migration of cancer cells"],"prefix":"10.1002","volume":"234","author":[{"given":"Nat\u00e1lia","family":"Barreto dos Santos","sequence":"first","affiliation":[{"name":"Departamento de Biologia Estrutural e Funcional Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Pires","family":"Bonfanti","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Estrutural e Funcional Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomaz Augusto Alves da","family":"Rocha\u2010e\u2010Silva","sequence":"additional","affiliation":[{"name":"Centro de Educa\u00e7\u00e3o em Sa\u00fade Abram Szajman, Faculdade Israelita de Ci\u00eancias da Sa\u00fade Albert Einstein S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Pedro Ismael","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Toxinologia Aplicada Instituto Butantan S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Alice","family":"da Cruz\u2010H\u00f6fling","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Biologia Tecidual Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liana","family":"Verinaud","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Estrutural e Funcional Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8804-497X","authenticated-orcid":false,"given":"Catarina","family":"Rap\u00f4so","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Estrutural e Funcional Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,5]]},"reference":[{"key":"e_1_2_9_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm5006463"},{"key":"e_1_2_9_1_3_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.14.6"},{"key":"e_1_2_9_1_4_1","first-page":"168","article-title":"Osteosarcoma cell proliferation and migration are partly regulated by redox\u2010activated NHE\u20101","volume":"1","author":"Bai H.","year":"2015","journal-title":"Journal of Clinical and\u00a0Translational Research"},{"key":"e_1_2_9_1_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652311795684722"},{"key":"e_1_2_9_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000362966"},{"key":"e_1_2_9_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-6389-0_1"},{"key":"e_1_2_9_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.08.054"},{"key":"e_1_2_9_1_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00503.2004"},{"key":"e_1_2_9_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2005.09.011"},{"key":"e_1_2_9_1_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000095135"},{"key":"e_1_2_9_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12423"},{"key":"e_1_2_9_1_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190902755505"},{"key":"e_1_2_9_1_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021836403433"},{"key":"e_1_2_9_1_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.10.5954"},{"key":"e_1_2_9_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_9_1_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-09-0207"},{"key":"e_1_2_9_1_18_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2005.46.5.597"},{"key":"e_1_2_9_1_19_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S106196"},{"key":"e_1_2_9_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2004.08.055"},{"key":"e_1_2_9_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.03.016"},{"key":"e_1_2_9_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-002-0623-8"},{"key":"e_1_2_9_1_23_1","doi-asserted-by":"publisher","DOI":"10.4314\/ajtcam.v11i5.17"},{"key":"e_1_2_9_1_24_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1740925X06000044"},{"key":"e_1_2_9_1_25_1","unstructured":"Minist\u00e9rio da Sa\u00fade. Instituto Nacional de C\u00e2ncer Jos\u00e9 Alencar Gomes da Silva (INCA) Estimativa. 2016 Incid\u00eancia de C\u00e2ncer no Brasil Rio de Janeiro RJ INCA 2015; Estimativa 2016: incid\u00eancia de c\u00e2ncer no Brasil\/Instituto Nacional de C\u00e2ncer Jos\u00e9 Alencar Gomes da Silva. Rio de Janeiro: INCA;2015."},{"key":"e_1_2_9_1_26_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.14.103"},{"key":"e_1_2_9_1_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-19-07674.2001"},{"issue":"2","key":"e_1_2_9_1_28_1","first-page":"233","article-title":"Scorpion and spider venoms in cancer treatment: State of the art, challenges, and perspectives","volume":"3","author":"Rap\u00f4so C.","year":"2017","journal-title":"Journal of Clinical and Translational Research"},{"key":"e_1_2_9_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2016.04.005"},{"key":"e_1_2_9_1_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0817-y"},{"key":"e_1_2_9_1_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107292"},{"key":"e_1_2_9_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.02.086"},{"key":"e_1_2_9_1_33_1","first-page":"1","article-title":"Improving the efficacy\u2010safety balance of polypharmacology in multi\u2010target drug discovery","volume":"12","author":"Ravikumar B.","year":"2017","journal-title":"Expert Opinion on Drug Discovery"},{"key":"e_1_2_9_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2017.03.021"},{"key":"e_1_2_9_1_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/02688697.2013.847170"},{"issue":"1","key":"e_1_2_9_1_36_1","first-page":"57","article-title":"Multimodality\u2013multiparametric brain tumors evaluation","volume":"20","author":"Tsitsia V.","year":"2017","journal-title":"Hellenic Journal of Nuclear Medicine"},{"key":"e_1_2_9_1_37_1","first-page":"1620","article-title":"Mechanisms for inhibition effects of polypeptide extract from scorpion venom (PESV) on proliferation of A549 cell lines in vitro","volume":"37","author":"Wang X.","year":"2012","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"e_1_2_9_1_38_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0708126"},{"key":"e_1_2_9_1_39_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.19484"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.26935","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26935","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.26935","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.26935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:30:45Z","timestamp":1777699845000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.26935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,5]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1002\/jcp.26935"],"URL":"https:\/\/doi.org\/10.1002\/jcp.26935","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,5]]},"assertion":[{"value":"2018-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}