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The current study cultured peritoneal mesothelial cells in high glucose to stimulate the environment of peritoneal fibrosis model in rats, and investigate whether microRNA\u201021 (miR\u201021) targeting Sprouty\u20101 affects high glucose\u2013induced fibrosis in peritoneal mesothelial cells via the rennin angiotensin system (Ras)\u2013mitogen\u2010activated protein kinase (MAPK) signaling pathway, as well as potential mechanisms. Peritoneal tissues in fibrosis rats were collected to extract peritoneal mesothelial cells, which, after in vitro culture, were transfected with a series of mimic or inhibitor of miR\u201021, or the small interfering RNA (siRNA) against Sprouty\u20101. Reverse\u2010transcription quantitative polymerase chain reaction and western blot analyses were performed to determine the levels of related genes or proteins. MTT assay and flow cytometry were conducted to observe the cell viability and cell apoptosis of peritoneal mesothelial cells. Dual\u2010luciferase reporter gene assay revealed that Sprouty\u20101 is the target gene of miR\u201021. Peritoneal fibrosis manifested\u00a0with elevated miR\u201021, extracellular\u2010signal\u2010regulated kinase (ERK), c\u2010Jun NH2\u2010terminal protein kinase (JNK), RAS and p38MAPK but reduced Sprouty\u20101. Cells transfected with miR\u201021 mimic exhibited decreased Sprouty\u20101 expressions, but increased levels of ERK, JNK, RAS, and p38MAPK. As for cellular process, miR\u201021 mimic or siRNA against Sprouty\u20101 exposure reduced cell viability, which resulted in more cells arrested at the G1 stage, and induced apoptosis. In contrast, miR\u201021 inhibitor exposure was observed to have induced effects on peritoneal mesothelial cells. These key findings provide evidence that miR\u201021 inhibits Sprouty\u20101 to promote the progression of fibrosis in peritoneal mesothelial cells by activating the Ras\u2013MAPK signaling pathway.<\/jats:p>","DOI":"10.1002\/jcp.26941","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T04:41:02Z","timestamp":1543984862000},"page":"5915-5925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>Retracted<\/i>: MicroRNA\u201021 contributes to high glucose\u2013induced fibrosis in peritoneal mesothelial cells in rat models by activation of the Ras\u2013MAPK signaling pathway via Sprouty\u20101"],"prefix":"10.1002","volume":"234","author":[{"given":"Qing","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Nephrology Zhongshan Hospital, Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Zhongshan Hospital, Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Zhongshan Hospital, Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9425-1641","authenticated-orcid":false,"given":"Tian\u2010Jun","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Nephrology Zhongshan Hospital, Xiamen University Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,4]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-012-0278-0"},{"issue":"2","key":"e_1_2_7_1_3_1","first-page":"50","article-title":"The role of microRNAs in cardiovascular disease","volume":"2","author":"Aghabozorg Afjeh S. 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