{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:50:36Z","timestamp":1765486236472,"version":"3.37.3"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,5]],"date-time":"2018-08-05T00:00:00Z","timestamp":1533427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Natural Sciences Foundation of Jiangsu Province","award":["BK20180596 recipient Xing Xie","BK20170600 recipient Maoda Pang","BK20160583","BK20140754"],"award-info":[{"award-number":["BK20180596 recipient Xing Xie","BK20170600 recipient Maoda Pang","BK20160583","BK20140754"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural and Science Foundation of China","doi-asserted-by":"crossref","award":["31700157","31400164","31700158"],"award-info":[{"award-number":["31700157","31400164","31700158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs by transfecting primary PBECs with the pEGFP\u2010hTERT recombinant plasmid containing human telomerase reverse transcriptase (hTERT). Immortalized PBECs (hTERT\u2010PBECs) retained the morphological and functional features of primary PBECs as indicated by cytokeratin 18 expression, telomerase activity assay, proliferation assays, karyotype analysis, and quantitative reverse\u2010transcriptase polymerase chain reaction. Compared to primary PBECs, hTERT\u2010PBECs had higher telomerase activity, extended replicative lifespan, and displayed enhanced proliferative activity. Moreover, this cell line is not transformed in vitro and does not exhibit a malignant phenotype in vivo, suggesting that it can be safely used in further studies. Besides, hTERT\u2010PBECs were susceptible to swine influenza virus of H3N2 subtype and porcine circovirus type 2. In conclusion, the immortalized hTERT\u2010PBECs represent a valuable in vitro model, which can be widely used in the study of porcine respiratory pathogenic infections.<\/jats:p>","DOI":"10.1002\/jcp.26942","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T06:58:47Z","timestamp":1533625127000},"page":"9763-9776","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Establishment and characterization of a telomerase\u2010immortalized porcine bronchial epithelial cell line"],"prefix":"10.1002","volume":"233","author":[{"given":"Xing","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences Nanjing 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