{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:48:13Z","timestamp":1790671693262,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Fundacja na rzecz Nauki Polskiej","doi-asserted-by":"publisher","award":["HOMING PLUS\/2010\u20102\/1"],"award-info":[{"award-number":["HOMING PLUS\/2010\u20102\/1"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>\n                    Liver, as one of the most important organs involved in lipids and glucose metabolism, is perceived as a key tissue for pharmacotherapy of insulin resistance (IRes) and type 2 diabetes. Ceramides (Cer) are biologically active lipids, which accumulation is associated with the induction of muscle IRes. We sought to determine the role of intrahepatic bioactive lipids production on insulin action in liver of insulin\u2010resistant rats and after myriocin administration. The experiments were conducted on male Wistar rats divided into three groups: Control, fed high\u2010fat diet (HFD), and fed HFD and treated with myriocin (HFD\/Myr). Before sacrifice, the animals were infused with a [U\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C]palmitate to calculate lipid synthesis rate by means of tracer incorporation technique in particular lipid groups. Liver Cer, diacylglycerols (DAG), acyl\u2010carnitine concentration, and isotopic enrichment were analyzed by LC\/MS\/MS. Proteins involved in lipid metabolism and insulin pathway were analyzed by western blot analysis. An OGTT and ITT was also performed. HFD\u2010induced IRes and increased both the synthesis rate and the content of DAG and Cer, which was accompanied by inhibition of an insulin pathway. Interestingly, myriocin treatment reduced synthesis rate not only of Cer but also DAG and improved insulin sensitivity. We conclude that the insulin\u2010sensitizing action of myriocin in the liver is a result of the lack of inhibitory effect of lipids on the insulin pathway, due to the reduction of their synthesis rate. This is the first study showing how the synthesis rate of individual lipid groups in liver changes after myriocin administration.\n                  <\/jats:p>","DOI":"10.1002\/jcp.27058","type":"journal-article","created":{"date-parts":[[2018,8,3]],"date-time":"2018-08-03T08:39:48Z","timestamp":1533285588000},"page":"1851-1861","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["The effect of high\u2010fat diet and inhibition of ceramide production on insulin action in liver"],"prefix":"10.1002","volume":"234","author":[{"given":"Piotr","family":"Zabielski","sequence":"first","affiliation":[{"name":"Department of Medical Biology Medical University of Bialystok Bialystok Poland"},{"name":"Department of Physiology Medical University of Bialystok Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaros\u0142aw","family":"Daniluk","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Internal Medicine Medical University of Bialystok Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hady Razak","family":"Hady","sequence":"additional","affiliation":[{"name":"1st Department of General Surgery and Endocrinology Medical University Bialystok Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam R.","family":"Markowski","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Gastroenterology Polish Red Cross Memorial Municipal Hospital Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Imierska","sequence":"additional","affiliation":[{"name":"Department of Hygiene Epidemiology and Metabolic Disorders, Medical University of Bialystok Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"G\u00f3rski","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical University of Bialystok Bialystok Poland"},{"name":"Department of Basic Sciences Faculty of Health Sciences, Lomza State University of Applied Sciences Lomza Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8055-0576","authenticated-orcid":false,"given":"Agnieszka U.","family":"Blachnio\u2010Zabielska","sequence":"additional","affiliation":[{"name":"Department of Physiology Medical University of Bialystok Bialystok Poland"},{"name":"Department of Hygiene Epidemiology and Metabolic Disorders, Medical University of Bialystok Bialystok Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,1]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/db17-1539"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22283"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000453174"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2012.126"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6216"},{"key":"e_1_2_7_1_7_1","article-title":"Ceramide metabolism is affected by obesity and diabetes in human adipose tissue","author":"Blachnio\u2010Zabielska A. U.","year":"2011","journal-title":"Journal of Cellular Physiology"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D035006"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.1.3"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2008.06.007"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90207.2008"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.37.6.667"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23621"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5300.665"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1311176110"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0750"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.84.5.1674"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)37163-7"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.686949"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800407-MCP200"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.01.002"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2007.04.014"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1103451108"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00117.2003"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1113359108"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advms.2016.04.003"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/154762"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.12331"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/980815"},{"issue":"1","key":"e_1_2_7_1_30_1","first-page":"72","article-title":"Blood volume in the rat","volume":"26","author":"Lee H. B.","year":"1985","journal-title":"Journal of Nuclear Medicine"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.23.2.0461"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-016-0194-7"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2011.00934.x"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.01.002"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.03.003"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R200009200"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.43.10.1203"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13478"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M008011"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI579"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313478200"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI30400"},{"key":"e_1_2_7_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.017"},{"key":"e_1_2_7_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(00)00110-8"},{"key":"e_1_2_7_1_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.24202"},{"key":"e_1_2_7_1_46_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10583"},{"key":"e_1_2_7_1_47_1","doi-asserted-by":"publisher","DOI":"10.1900\/RDS.2008.5.13"},{"key":"e_1_2_7_1_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-007-0781-2"},{"key":"e_1_2_7_1_49_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.5.1215"},{"key":"e_1_2_7_1_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406499200"},{"key":"e_1_2_7_1_51_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D500026-JLR200"},{"key":"e_1_2_7_1_52_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc06-1539"},{"key":"e_1_2_7_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44844-7"},{"key":"e_1_2_7_1_54_1","doi-asserted-by":"publisher","DOI":"10.5604\/01.3001.0011.7380"},{"key":"e_1_2_7_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2013.08.013"},{"key":"e_1_2_7_1_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.91014.2008"},{"key":"e_1_2_7_1_57_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-16-0381"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.27058","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27058","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcp.27058","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.27058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:32:49Z","timestamp":1777699969000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.27058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,1]]},"references-count":56,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1002\/jcp.27058"],"URL":"https:\/\/doi.org\/10.1002\/jcp.27058","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,1]]},"assertion":[{"value":"2018-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}