{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:28:34Z","timestamp":1773293314198,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T00:00:00Z","timestamp":1536278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006485","name":"National Institute of Genetic Engineering and Biotechnology","doi-asserted-by":"crossref","award":["940501-I-509"],"award-info":[{"award-number":["940501-I-509"]}],"id":[{"id":"10.13039\/501100006485","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epigenetic reprogramming by embryonic stem cell\u2010specific miR\u2010302\/367 cluster has shown some tumor suppressive effects in cancer cells of different tissues such as skin, colon, and cervix. Vitamin C has been known as a reprogramming enhancer of human and mouse somatic cells. In this study, first we aimed to investigate whether exogenous induction of miR\u2010302\/367 in breast cancer cells shows the same tumor suppressive effects previously observed in other cancer cells lines, and whether vitamin C can enhance reprogramming of breast cancer cells and also improve the tumor suppressive function of miR\u2010302\/367 cluster. Overexpression of miR\u2010302\/367 cluster in MDA\u2010MB\u2010231 and SK\u2010BR\u20103 breast cancer cells upregulated expression of miR\u2010302\/367 members and also some core pluripotency factors including \n<jats:italic>OCT4A<\/jats:italic>, \n<jats:italic>SOX2<\/jats:italic> and \n<jats:italic>NANOG<\/jats:italic>, induced mesenchymal to epithelial transition, suppressed invasion, proliferation, and induced apoptosis in the both cell lines. However, treatment of the miR\u2010302\/367 transfected cells with vitamin C suppressed the expression of pluripotency factors and augmented the tumorigenicity of the breast cancer cells by restoring their proliferative and invasive capacity and compromising the apoptotic effect of miR\u2010302\/367. Supplementing the culture medium with vitamin C downregulated expression of \n<jats:italic>TET1<\/jats:italic> gene which seems to be the reason behind the negative impact of vitamin C on the reprogramming efficiency of miR\u2010302\/367 cluster and its anti\u2010tumor effects. Therefore application of vitamin C may not always serve as a reprogramming enhancer depending on its switching function on \n<jats:italic>TET1<\/jats:italic>. This phenomenon should be carefully considered when considering a reprogramming strategy for tumor suppression.<\/jats:p>","DOI":"10.1002\/jcp.27081","type":"journal-article","created":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T12:13:29Z","timestamp":1536322409000},"page":"2672-2682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Vitamin C counteracts miR\u2010302\/367\u2010induced reprogramming of human breast cancer cells and restores their invasive and proliferative capacity"],"prefix":"10.1002","volume":"234","author":[{"given":"Bahareh","family":"Ramezankhani","sequence":"first","affiliation":[{"name":"Department of Stem Cells and Regenerative Medicine Institute for Medical Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7492-413X","authenticated-orcid":false,"given":"Masoumeh F.","family":"Taha","sequence":"additional","affiliation":[{"name":"Department of Stem Cells and Regenerative Medicine Institute for Medical Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1064-0373","authenticated-orcid":false,"given":"Arash","family":"Javeri","sequence":"additional","affiliation":[{"name":"Department of Stem Cells and Regenerative Medicine Institute for Medical Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB) Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,7]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.03.001"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00398-08"},{"issue":"106","key":"e_1_2_8_1_4_1","first-page":"e53486","article-title":"Imaging\u2010 and flow cytometry\u2010based analysis of cell position and the cell cycle in 3d melanoma spheroids","author":"Beaumont K. 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