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Meanwhile, we previously reported that miR\u2010483\u20105p is essential for the initiation and development of OA by stimulating chondrocyte hypertrophy and angiogenesis. The connection between mTORC1 and miR\u2010483\u20105p activation in OA progression, however, remains unclear. In this study, we elucidated their relationship and identified the underlying mechanisms. The expression of miR\u2010483\u20105p in the articular cartilage of\u00a0cartilage\u2010specific TSC1 knockout mice was assessed compared with control mice using the Agilent Mouse miRNA (8*60K) V19.0 array and real\u2010time polymerase chain reaction (RT\u2010PCR). The functional effects of the stimulation of miR\u2010483\u20105p via histone deacetylase 4 (HDAC4) by mTORC1 in OA development, subsequently modulating its downstream targets\u00a0matrilin 3 and tissue inhibitor of metalloproteinase 2, were examined by immunostaining, western blotting, and real\u2010time PCR. This study revealed that miR\u2010483\u20105p was responsible for mTORC1 activation\u2010stimulated OA. Mechanistically, mTORC1 controls the HDAC4\u2010dependent expression of miR\u2010483\u20105p to stimulate chondrocyte hypertrophy, extracellular matrix degradation, and subchondral bone angiogenesis, and it consequently initiates and accelerates the development of OA. Our findings revealed a novel mTORC1\u2010HDAC4\u2010miR\u2010483\u20105p pathway that is critical for OA development.<\/jats:p>","DOI":"10.1002\/jcp.27088","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T04:30:54Z","timestamp":1535430654000},"page":"2730-2740","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Chondrocyte mTORC1 activation stimulates miR\u2010483\u20105p via HDAC4 in osteoarthritis progression"],"prefix":"10.1002","volume":"234","author":[{"given":"Hua","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Tropical Diseases and Translational Medicine of the Ministry of Education &amp; Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College Haikou China"},{"name":"Department of Cell Biology School of Basic Medical Science, Southern Medical University Guangzhou 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