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Long noncoding RNA (lncRNA) metastasis\u2010associated lung adenocarcinoma transcript 1 (MALAT1) is increased in patients with MI. However, the functions of MALAT1 in cardiac fibrosis have not been elucidated. This study elucidates the roles of MALAT1 in MI and the underlying mechanisms. The MI model was established by artificial coronary artery occlusion in mice. Western blot analysis and quantitative reverse transcription\u2010polymerase chain reaction were performed to analyze protein expression and RNA expression, respectively. Cardiac function was measured by echocardiography. Masson\u2019s trichrome staining was used to exhibit the fibrotic area in MI hearts. Cardiac fibroblasts were isolated from newborn pups, and cell proliferation was determined by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide assay. Upregulation of MALAT1 and downregulation of microRNA\u2010145 (miR\u2010145) were induced in MI heart and angiotensin II (AngII)\u2010treated cardiac fibroblasts, and the inhibition of miR\u2010145 expression was reversed by MALAT1 depletion. Knockdown MALAT1 ameliorated MI\u2010impaired cardiac function and prevented AngII\u2010induced fibroblast proliferation, collagen production, and \u03b1\u2010SMA expression in cardiac fibroblasts. MALAT1 stability and transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) activity were regulated by miR\u2010145. AngII\u2010induced TGF\u2010\u03b21 activity in cardiac fibroblasts was blocked by MALAT1 knockdown. Based on these results, we concluded that lncRNA MALAT1 promotes cardiac fibrosis and deteriorates cardiac function post\u2010MI by regulating TGF\u2010\u03b21 activity via miR\u2010145.<\/jats:p>","DOI":"10.1002\/jcp.27117","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T05:52:54Z","timestamp":1535435574000},"page":"2997-3006","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":97,"title":["Long noncoding RNA MALAT1 mediates cardiac fibrosis in experimental postinfarct myocardium mice model"],"prefix":"10.1002","volume":"234","author":[{"given":"Songqun","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Cardiology Changhai Hospital, Second Military Medical University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Changhai Hospital, Second 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