{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:04:19Z","timestamp":1761663859437,"version":"3.41.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,8,26]],"date-time":"2018-08-26T00:00:00Z","timestamp":1535241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770638","81170351"],"award-info":[{"award-number":["81770638","81170351"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute cholecystitis is a common disease with gallbladder dysmotility. Disease pathogenesis involves immune cell infiltration as well as changes in gallbladder interstitial Cajal\u2010like cells (ICLCs). However, it remains unclear if or how the immune cells affect ICLC morphology, density, distribution, and function in gallbladder tissue during acute cholecystitis. In this study, we explored the acute cholecystitis\u2010related alterations in gallbladder ICLCs in a guinea pig model, focusing on the effects of neighboring neutrophils. Adult guinea pigs were randomly divided into four groups (control, 24\u2009hr common bile duct ligation [CBDL], 48\u2010hr CBDL, and antipolymorphonuclear neutrophil [PMN] treated) and analyzed using methylene blue staining and immunofluorescence. Gallbladder contractility was also monitored. To culture gallbladder ICLCs, collagenase digestion was performed on tissue from 10\u2010 to 15\u2010day\u2010old guinea pigs. Neutrophils isolated from the peripheral blood of experimental animals 48\u2010hr postsurgery were also cocultured with the gallbladder ICLCs. Intracellular calcium was detected with Fluo\u20104 AM dye. Our results showed that gallbladder ICLC density significantly declined during acute cholecystitis and was accompanied by shortening of the cellular processes and damage to their network\u2010like structure. However, pretreatment with anti\u2010PMN partially prevented these changes. Gallbladder contraction was also significantly decreased during acute cholecystitis, and this appeared to be mediated by the neutrophils. Moreover, ICLCs cocultured with neutrophils also had shortened and reduced processes and impaired network\u2010like structure formation. Intracellular calcium transient was less sensitive to contraction agonists and inhibitors when cocultured with neutrophils. Taken together, neutrophils greatly affect gallbladder ICLCs and dysmotility during acute cholecystitis.<\/jats:p>","DOI":"10.1002\/jcp.27197","type":"journal-article","created":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T08:15:33Z","timestamp":1546416933000},"page":"4291-4301","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Neutrophils injure gallbladder interstitial Cajal\u2010like cells in a guinea pig model of acute cholecystitis"],"prefix":"10.1002","volume":"234","author":[{"given":"Meng\u2010Juan","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan China"},{"name":"Key Laboratory of Hubei Province for Digestive System Diseases Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan China"},{"name":"Key Laboratory of Hubei Province for Digestive System Diseases Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010Peng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan China"},{"name":"Key Laboratory of Hubei Province for Digestive System Diseases Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan China"},{"name":"Key Laboratory of Hubei Province for Digestive System Diseases Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4855-7065","authenticated-orcid":false,"given":"Bao\u2010Ping","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan China"},{"name":"Key Laboratory of Hubei Province for Digestive System Diseases Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,26]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12545"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2015.09.010"},{"key":"e_1_2_8_1_4_1","first-page":"217","article-title":"Sur le ganglions et plexus nerveux de l\u2019intestin","volume":"5","author":"Cajal S. 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