{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:03:02Z","timestamp":1790866982078,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,28]],"date-time":"2018-10-28T00:00:00Z","timestamp":1540684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 105\u20102628\u2010B\u2010197\u2010001\u2010MY3"],"award-info":[{"award-number":["MOST 105\u20102628\u2010B\u2010197\u2010001\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 106\u20102811\u2010B\u2010197\u2010001"],"award-info":[{"award-number":["MOST 106\u20102811\u2010B\u2010197\u2010001"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dysregulation of NACHT, LRR, and PYD domains\u2010containing protein 3 (NLRP3) inflammasome is involved in many chronic inflammatory diseases, including gouty arthritis. Activation of the NLRP3 inflammasome requires priming and activation signals: the priming signal controls the expression of NLRP3 and interleukin (IL)\u20101\u03b2 precursor (proIL\u20101\u03b2), while the activation signal leads to the assembly of the NLRP3 inflammasome and to caspase\u20101 activation. Here, we reported the effects of the alcoholic extract of Taiwanese green propolis (TGP) on the NLRP3 inflammasome in vitro and in vivo. TGP inhibited proIL\u20101\u03b2 expression by reducing nuclear factor kappa B activation and reactive oxygen species (ROS) production in lipopolysaccharide\u2010activated macrophages. Additionally, TGP also suppressed the activation signal by reducing mitochondrial damage, ROS production, lysosomal rupture, c\u2010Jun N\u2010terminal kinases 1\/2 phosphorylation and apoptosis\u2010associated speck\u2010like protein oligomerization. Furthermore, we found that TGP inhibited the NLRP3 inflammasome partially via autophagy induction. In the in vivo mouse model of uric acid crystal\u2010induced peritonitis, TGP attenuated the peritoneal recruitment of neutrophils, and the levels of IL\u20101\u03b2, active caspase\u20101, IL\u20106 and monocyte chemoattractant protein\u20101 in lavage \ufb02uids. As a proof of principle, in this study, we purified a known compound, propolin G, from TGP and identified this compound as a potential inhibitor of the NLRP3 inflammasome. Our results indicated that TGP might be useful for ameliorating gouty inflammation via inhibition of the NLRP3 inflammasome.<\/jats:p>","DOI":"10.1002\/jcp.27204","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T04:59:22Z","timestamp":1540789162000},"page":"4081-4094","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Mechanistic insight into the attenuation of gouty inflammation by Taiwanese green propolis via inhibition of the NLRP3 inflammasome"],"prefix":"10.1002","volume":"234","author":[{"given":"Chih\u2010Yu","family":"Hsieh","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Animal Science National Ilan University Yilan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan\u2010Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Linsen Chinese Medicine and Kunming Branch, 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