{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T09:09:02Z","timestamp":1772010542002,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,8,21]],"date-time":"2018-08-21T00:00:00Z","timestamp":1534809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81741123"],"award-info":[{"award-number":["81741123"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572093"],"award-info":[{"award-number":["81572093"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Suppressed osteogenic differentiation is considered a main cause of ethanol\u2010induced osteonecrosis. Tumor necrosis factor \u03b1 (TNF\u2010\u03b1) and miR\u201031 have been reported to be involved in the osteogenic induction. This study aimed to explore a possible molecular mechanism regulating osteogenic differentiation in ethanol\u2010induced osteonecrosis bone marrow stromal stem cells (BMSCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Alizarin red staining was used to examine the level of mineralization in osteogenic differentiation process. Alkaline phosphatase assay was applied to the validation of ALP level which was essential to bone mineralization. The level of osteogenesis markers was determined by western blot assay, whereas the fluctuations of messenger RNA levels were tested by quantitative real\u2010time polymerase chain reaction. Microarray analysis was conducted to identify differentially expressed genes, because the possible target relationship was predicted and validated by miRBase and luciferase reporter assay, respectively. Colony forming unit of fibroblast assay was used to observe the proliferation of BMSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>BMSCs from patients with ethanol\u2010induced osteonecrosis exhibited weaker osteogenic differentiation and proliferation abilities. TNF\u2010\u03b1 inhibitor added in the osteogenic medium significantly enhanced the osteogenic differentiation ability and BMSCs proliferation ability. TNF\u2010\u03b1 by regulating miR\u201031 downregulated the expressions of RUNX2 and SATB2, two contributors of osteoblast differentiation, further suppressed osteogenic differentiation. On the contrary, TNF\u2010\u03b1 inhibitor could promote osteogenic differentiation in BMSCs from patients with ethanol\u2010induced osteonecrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TNF\u2010\u03b1 inhibitor could downregulate miR\u201031 expressions, which directly promoted SATB2 and RUNX2 expressions and enhanced osteogenic differentiation of BMSCs from patients with ethanol\u2010induced osteonecrosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27210","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T16:10:45Z","timestamp":1534954245000},"page":"4314-4326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Decrease of MiR\u201031 induced by TNF\u2010\u03b1 inhibitor activates SATB2\/RUNX2 pathway and promotes osteogenic differentiation in ethanol\u2010induced osteonecrosis"],"prefix":"10.1002","volume":"234","author":[{"given":"Lingjia","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yisheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Orthopedics Department Guangdong Provincial Hospital of Traditional Chinese Medicine Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery 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