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The goal was to investigate the hypothesis that TNF\u2010\u03b1 from abdominal adipose tissue (adipocytes and macrophages) would decrease sugar and amino acid transport acting from the basolateral membrane of the enterocytes. TNF\u2010\u03b1 placed in the basal compartment of Caco\u20102 cells decreased \u03b1\u2010methyl\u2010\n<jats:sc>d<\/jats:sc>\u2010glucose (\u03b1MG) and glutamine uptake. The apical medium derived from these Caco\u20102 cells apically placed in another set of cells, also reduced sugar and glutamine transport. Reverse\u2010transcription polymerase chain reaction analysis demonstrated upregulation of <jats:italic>TNF\u2010\u03b1<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic>, and <jats:italic>MCP1<\/jats:italic> expression in Caco\u20102 cells exposed to basal TNF\u2010\u03b1. Similarly, \u03b1MG uptake was inhibited after Caco\u20102 cells were incubated, in the basal compartment, with medium from visceral human mesenchymal stem cells\u2010derived adipocytes of overweight individuals. The apical medium collected from those Caco\u20102 cells, and placed in the upper side of other set of cells, also decreased sugar uptake. Basal presence of medium derived from lipopolysaccharide\u2010activated macrophages and nonactivated macrophages decreased \u03b1MG uptake as well. Diet\u2010induced obese mice showed an increase in the visceral adipose tissue surrounding the intestine. In this physiological condition, there was a reduction on \u03b1MG uptake in jejunal everted rings.<\/jats:p><jats:p>Altogether, these results suggest that basolateral TNF\u2010\u03b1, which can be produced by adipocytes and macrophages during obesity, would be able to activate TNF\u2010\u03b1 and other proinflammatory proteins expression in the small intestine and diminish intestinal sugar and amino acids transport.<\/jats:p>","DOI":"10.1002\/jcp.27216","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T08:51:15Z","timestamp":1537779075000},"page":"4352-4361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Basolateral presence of the proinflammatory cytokine tumor necrosis factor \u2010\u03b1 and secretions from adipocytes and macrophages reduce intestinal sugar 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