{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:03:22Z","timestamp":1759939402608,"version":"3.41.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T00:00:00Z","timestamp":1538006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Alterations in the nasal epithelial barrier homeostasis and increased interleukin 33 (IL\u201033) expression contribute to the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>As Notch\u20101 signaling is crucial in repair processes of mucosa, the current study assessed Notch\u20101\/Jagged\u20101 signaling and IL\u201033 in the epithelium of nasal polyps biopsies from allergic (A\u2010CRSwNP; \n<jats:italic>n<\/jats:italic>\u2009=\u20099) and not allergic (NA\u2010CRSwNP; \n<jats:italic>n<\/jats:italic>\u2009=\u20099) subjects by immunohistochemistry. We also assessed, in a model of nasal epithelial cells, the effects of stimulation of Notch\u20101 with Jagged\u20101 on the expression of IL\u201033 (by flow cytometry, immunofluorescence, and immunocytochemistry), Jagged\u20101 (by flow cytometry), and\u00a0p\u2010CREB transcription factor (by western blot analysis).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ex vivo\u00a0(a) in normal epithelium, the expression of Notch\u20101 and IL\u201033 were higher in NA\u2010CRSwNP than in A\u2010CRSwNP; (b) in metaplastic epithelium, the expression of Notch\u20101, Jagged\u20101, and IL\u201033 were higher in NA\u2010CRSwNP than in A\u2010CRSwNP; (c) in hyperplastic epithelium, the expression of Notch\u20101, Jagged\u20101, and IL\u201033 were higher in A\u2010CRSwNP than in NA\u2010CRSwNP; and (d) in basal epithelial cells, no differences were observed in the expression of Jagged\u20101, IL\u201033, and Notch\u20101. The expression of Notch\u20101 significantly correlated with the expression of IL\u201033. In vitro, stimulation of Notch\u20101 with Jagged\u20101 induced the expression of (a) Jagged\u20101; (b) IL\u201033; and (c) p\u2010CREB transcription factor. The inhibitor of Notch\u20101, DAPT, reduced all the effects of Jagged\u20101 on nasal epithelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data herein provided support, for the first time, a putative role of Notch\u20101\/Jagged\u20101 signaling in the overexpression of IL\u201033 in the epithelium of nasal polyps from patients with CRSwNP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27237","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T10:10:29Z","timestamp":1538043029000},"page":"4582-4596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Notch\u20101 signaling activation sustains overexpression of interleukin 33 in the epithelium of nasal polyps"],"prefix":"10.1002","volume":"234","author":[{"given":"G.","family":"Chiappara","sequence":"first","affiliation":[{"name":"Istituto di Biomedicina e Immunologia Molecolare, Dipartimento 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