{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T18:13:12Z","timestamp":1783275192691,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Shandong Natural Science Foundation","award":["ZR2012HM082"],"award-info":[{"award-number":["ZR2012HM082"]}]},{"name":"Shandong Natural Science Foundation","award":["ZR2016GB11"],"award-info":[{"award-number":["ZR2016GB11"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>The aim of our study was to validate the sway of\u00a0long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) on the metabolism and growth of bladder cancer cells by microRNA\u201031 (miR\u201031)\/cyclin\u2010dependent kinase 1 (\n<jats:italic>CDK1<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The Gene Expression Omnibus database was used for analyzing the differentially expressed lncRNA and messenger RNA (mRNA) in bladder cancer tissues, with the highly expressed lncRNA PVT1 and mRNA <jats:italic>CDK1<\/jats:italic> screened out. The expression level of PVT1 was detected by\u00a0quantitative reverse\u2010transcription polymerase chain reaction, cell viability by Cell Counting Kit\u20108 assay, cell proliferation and scratch by 5\u2010bromo\u20102\u2032\u2010deoxyuridine assay, cell migration and invasion by transwell assays, the expression level of \n<jats:italic>CDK1<\/jats:italic> by immunohistochemistry and western blot analysis, transcription factor targeting by dual\u2010luciferase assay, and the effect of PVT1 on bladder cancer growth by nude mice tumor formation experiment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LncRNA PVT1 and mRNA <jats:italic>CDK1<\/jats:italic> had a higher expression in bladder cancer cells than that in neighboring tissues. Activity, proliferation, colony formation, migration, and invasion of bladder cancer cell were noticeably reduced by the PVT1 inhibitor than that of control group. PVT1 and \n<jats:italic>CDK1<\/jats:italic> have binding sites with miR\u201031. When miR\u201031 decreased, CDK1 mRNA and protein levels increased in vivo experiments in nude mice. When PVT1 was downregulated, the tumor size was significantly reduced and tumor proliferation was curbed. Immunohistochemistry showed that the positive rate of \n<jats:italic>CDK1<\/jats:italic> and \n<jats:italic>Ki\u201067<\/jats:italic> decreased and the expression of miR\u201031 increased after PVT1 was inhibited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>LncRNA PVT1 was overexpressed in bladder cancer cells, and it was downregulated miR\u201031 to enhance <jats:italic>CDK1<\/jats:italic> expression and facilitate bladder cancer cells proliferation, migration, and invasion.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27279","type":"journal-article","created":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T10:10:46Z","timestamp":1539511846000},"page":"4799-4811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":88,"title":["LncRNA PVT1 regulates growth, migration, and invasion of bladder cancer by 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