{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T22:38:26Z","timestamp":1776811106361,"version":"3.51.2"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T00:00:00Z","timestamp":1536192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002958","name":"Drug Applied Research Center, Tabriz University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recurrent pregnancy loss (RPL) is a multifactorial disorder of women in reproductive age, which in some cases is caused by immunologic abnormalities. In this study, we aimed to evaluate cellular and molecular components of the immune system like different T\u2010cell subsets and their regulating microRNAs (miRNAs) in RPL women and control group. Fifty RPL and 50 healthy subjects were recruited. Subsets of T cells, including regulatory T (Treg) cells, helper T (Th) 17 cells, exhausted T cells, exhausted Treg cells were evaluated by flow cytometry. Transcription factors of T cells and related miRNA profile were quantified using real\u2010time polymerase chain reaction (RT\u2010PCR). Assessment showed that Treg and exhausted T cells, were decreased in RPL patients (<jats:italic>p<\/jats:italic>\u2009=\u20090.009 and 0.02, respectively), while an increase was observed in Th17 and exhausted Treg frequency (\n<jats:italic>p<\/jats:italic>\u2009=\u20090.013 and 0.0037, respectively). Messenger RNA expression level of \n<jats:italic>T\u2010bet<\/jats:italic> and \n<jats:italic>IRF4<\/jats:italic> was upregulated in RPL patients (\n<jats:italic>p<\/jats:italic>\u2009=\u20090.011 and 0.0001, respectively), while Th2\u2010 and Treg\u2010related transcription factors, \n<jats:italic>GATA3<\/jats:italic> and \n<jats:italic>GITR<\/jats:italic>, were downregulated in these patients compared with the healthy subjects (\n<jats:italic>p<\/jats:italic>\u2009=\u20090.0008 and &lt;0.000, respectively). Treg\u2010associated miRNAs, the miR\u2010106b\u201025\u201393 cluster, showed a higher rate in RPL patients (\n<jats:italic>P<\/jats:italic>\u2009=\u20090.007, 0.001, and 0.029, respectively), however, we observed no significant difference in the expression level of Th17\u2010associated miRNA, mir\u2010326. According to the results, we concluded that unbalanced immune responses and deregulated function of T\u2010cell subsets may lead to reproduction\u2010related failure like a miscarriage. Therefore, evaluation of immune cells and related miRNA profile may serve as prognostic biomarker for the treatment of RPL patients.<\/jats:p>","DOI":"10.1002\/jcp.27290","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T09:02:35Z","timestamp":1536224555000},"page":"4924-4933","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Altered T\u2010cell subpopulations in recurrent pregnancy loss patients with cellular immune abnormalities"],"prefix":"10.1002","volume":"234","author":[{"given":"Samaneh","family":"Abdolmohammadi Vahid","sequence":"first","affiliation":[{"name":"Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Student\u2019s Research Committee, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahnaz","family":"Ghaebi","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Student\u2019s Research Committee, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5367-9956","authenticated-orcid":false,"given":"Mohammad","family":"Nouri","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences Tabriz 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Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0099-6728","authenticated-orcid":false,"given":"Mehdi","family":"Yousefi","sequence":"additional","affiliation":[{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Aging Research Institute, Tabriz University of Medical Sciences Tabriz 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