{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T23:15:47Z","timestamp":1793229347735,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Mesenchymal stem cells (MSCs)\u2010based regenerative therapy is now considered as an alternative approach to revive infectious diseases, including sepsis. Nevertheless, the efficiency of MSC application is limited by the poor survival rate of engrafted MSCs. Hence, preconditioning was established as a strategy to increase the cells\u2019 efficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MSCs were preconditioned with 1\u2009\u03bcg\/ml of three different lipopolysaccharides (LPSs) of \n<jats:italic>Pseudomonas<\/jats:italic> (Pse\u2010LPS), \n<jats:italic>Acinetobacter<\/jats:italic> (Ac\u2010LPS), and \n<jats:italic>Acinetobacter<\/jats:italic> inactivated lipid A by PagL (Ac\u2010LPS\u2010PagL). Then, preconditioned MSCs were exposed to oxidative stress and serum deprivation followed by evaluation of the antibacterial activity, survival, and apoptosis of MSCs. Then, the murine sepsis model treated with 100\u2009\u03bcl phosphate\u2010buffered saline (control group, sepsis group), 100\u2009\u03bcl of 1\u2009\u00d7\u200910\n<jats:sup>6<\/jats:sup> wild MSCs (MSC group), and three remained groups received 100\u2009\u03bcl of 1\u2009\u00d7\u200910\n<jats:sup>6<\/jats:sup> LPS\u2010preconditioned MSCs (Pse\u2010LPS\u2010MSCs group: LPS purified from \n<jats:italic>Pseudomonas<\/jats:italic>, or Ac\u2010LPS\u2010MSCs group: LPS purified from \n<jats:italic>Acinetobacter<\/jats:italic>, and Ac\u2010PagL\u2010LPS\u2010MSCs group: detoxified LPS Pagl).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 4 days, LPS\u2010preconditioned MSC transplantation modulated the immune response and reduced inflammation in septic mice. Apoptosis of Pse\u2010LPS\/Ac\u2010LPS\u2010preconditioned\u2010MSCs was obviously reduced in vitro, and the survival rate of engrafted mice was evidently elevated in Pse\u2010LPS\u2010MSCs and Ac\u2010LPS\u2010MSCs groups compared with other three groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LPS preconditioning provides an innovative strategy for evolving functional and biological properties of MSCs and ameliorates the survival rate of the mouse model of sepsis after MSC transplantation, protects cells from apoptosis and organ damages, and evaluates therapeutic properties, including immunemodulatory.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27298","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T00:14:23Z","timestamp":1537056863000},"page":"4970-4986","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Antimicrobial effects of mesenchymal stem cells primed by modified LPS on bacterial clearance in sepsis"],"prefix":"10.1002","volume":"234","author":[{"given":"Pardis","family":"Saeedi","sequence":"first","affiliation":[{"name":"Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3363-8276","authenticated-orcid":false,"given":"Raheleh","family":"Halabian","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas Ali Imani","family":"Fooladi","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,14]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0192-0"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-014-0560-1"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000371815"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2012.09.004"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002150"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096161"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2369"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3584-0_7"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2014.70"},{"key":"e_1_2_6_1_12_1","first-page":"1","article-title":"Osteogenic differentiation of pre\u2010conditioned bone marrow mesenchymal stem cells with lipopolysaccharide on modified poly\u2010L\u2010lactic\u2010acid nanofibers","author":"Kooshki H.","year":"2018","journal-title":"Journal of Cellular Physiology"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149876"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3723"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.16-0415"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.12.014"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.544"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.17850"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201307-1272LE"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt499"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2017.17.2.89"},{"key":"e_1_2_6_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-017-1042-6"},{"key":"e_1_2_6_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.08.013"},{"key":"e_1_2_6_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808146106"},{"key":"e_1_2_6_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cppeds.2013.10.001"},{"key":"e_1_2_6_1_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3924858"},{"key":"e_1_2_6_1_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0363-7"},{"issue":"4","key":"e_1_2_6_1_29_1","article-title":"The effects of preconditioning on survival of mesenchymal stem cells in vitro","volume":"3","author":"Taban Z. 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