{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:52:21Z","timestamp":1788537141157,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The research aimed at detecting the autophagy level of osteoblast in osteoporosis rat, and investigating the effect of autophagy gene damage\u2010regulated autophagy modulator (<jats:italic>DRAM<\/jats:italic>) on osteoblast proliferation, cell cycle, apoptosis, and autophagy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The level of osteocalcin (OCN) and C\u2010telopeptide (CTX) in serum of ovariectomized (OVX) rats was detected by enzyme\u2010linked immunosorbent assay (ELISA). The Oil Red\u2010O staining was used to observing bone histological changes. The messenger RNA level and protein expression level of Runt\u2010related transcription factor 2 (<jats:italic>Runx2<\/jats:italic>; osteoblast markers) and other autophagy\u2010related genes were revealed using quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) and western blot. The changes of autophagy in osteoblasts were detected by immunofluorescence staining. The following experiments were performed in osteoblasts of OVX rats through transfected with silencing \n<jats:italic>DRAM<\/jats:italic> to detecting cell proliferation, cell cycle, and apoptosis by Cell Counting Kit\u20108 assays and flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The result of ELISA showed a significantly elevated of OCN and CTX in OVX rats as well a high fat content compared with sham\u2010operated\u00a0rats. The expression of Runx2 in bone of proximal tibia was higher by qRT\u2010PCR and western blot analysis. The immunofluorescence staining and transmission electron microscope observe revealed that pcDNA3\u2010<jats:italic>DRAM<\/jats:italic> could promote the autophagy in OVX rats. Besides that, overexpression of \n<jats:italic>DRAM<\/jats:italic> inhibited cell proliferation, promoted apoptosis, and enhanced autophagy in osteoblasts. The results of Oil Red\u2010O staining indicated that overexpression of DRAM enhanced lipid accumulation in osteoporosis rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The autophagy level of OVX rats was weakened, but overexpressed <jats:italic>DRAM<\/jats:italic> could increase the autophagy level of osteoblast, suppress proliferation, and induce apoptosis of osteoblast.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27304","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T06:42:02Z","timestamp":1536648122000},"page":"5023-5032","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effect of autophagy gene <i>DRAM<\/i> on proliferation, cell cycle, apoptosis, and autophagy of osteoblast in osteoporosis rats"],"prefix":"10.1002","volume":"234","author":[{"given":"Ning","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Chinese Academy of Medical Sciences and Peking Union Medical College Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3852-4873","authenticated-orcid":false,"given":"Hong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Chinese Academy of Medical Sciences and Peking Union Medical College Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Chinese Academy of Medical Sciences and Peking Union Medical College Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulei","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Chinese Academy of Medical Sciences and Peking Union Medical College Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,10]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/jwh.2008.0897"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12013"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-014-1194-1"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.05.034"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4752"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.08.016"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.17552"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/1806-9282.63.02.173"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092260403"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2013.147"},{"issue":"2","key":"e_1_2_7_1_12_1","first-page":"115","article-title":"Management of postmenopausal osteoporosis and the prevention of fractures","volume":"56","author":"Gambacciani M.","year":"2014","journal-title":"Panminerva Medica"},{"issue":"5","key":"e_1_2_7_1_13_1","first-page":"635","article-title":"Calcium and bone metabolism across women\u2019s life stages. 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