{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:03:22Z","timestamp":1790208202248,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,11,23]],"date-time":"2018-11-23T00:00:00Z","timestamp":1542931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372081"],"award-info":[{"award-number":["81372081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore the effectiveness of the insulin\u2010like growth factor 1\u00a0receptor (IGF\u20101R)\/PI3K\/AKT pathway in promoting the synthesis of the auricular chondrocyte extracellular matrix (ECM) using concentrated growth factor (CGF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Chondrocytes isolated from the remnant auricular cartilage of microtia patients were randomly divided into different experimental and control groups, then stimulated with a reagent.\u00a0IGF\u20101 released by CGF was quantified by enzyme\u2010linked immunosorbent assay. Glycosaminoglycan (GAG), proteoglycan, and type II collagen (COLAII) were examined by histological and immunohistological analysis. Expression levels of IGF\u20101R, pIGF\u20101R, PI3K, pPI3K, AKT, pAKT, COLAII, and Aggrecan were detected by western blot analysis technique and\u00a0gene expression was tested by real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CGF significantly stimulated the synthesis of COLAII and Aggrecan and increased the phosphorylation levels of IGF\u20101R, PI3K, and AKT. Small interfering RNA IGF\u20101 blocked ECM synthesis, COLAII and Aggrecan gene expression, and IGF\u20101R\/PI3K\/AKT activation. Inhibitor AG1024 and LY294002 significantly inhibited ECM synthesis and the phosphorylation of IGF\u20101R, PI3K, and AKT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CGF\u2010released IGF\u20101 stimulates the synthesis of the auricular chondrocyte ECM via the IGF\u20101R\/PI3K\/AKT signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27316","type":"journal-article","created":{"date-parts":[[2018,11,24]],"date-time":"2018-11-24T15:01:36Z","timestamp":1543071696000},"page":"21817-21824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Microtia patients: Auricular chondrocyte ECM is promoted by CGF through IGF\u20101 activation of the IGF\u20101R\/PI3K\/AKT pathway"],"prefix":"10.1002","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6674-1619","authenticated-orcid":false,"given":"Xia","family":"Chen","sequence":"first","affiliation":[{"name":"Department of 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