{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T18:07:53Z","timestamp":1770487673926,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T00:00:00Z","timestamp":1540512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cancer is one of the common lifestyle diseases and is considered to be the leading cause of death worldwide. Epstein\u2013Barr virus (EBV)\u2010infected individuals remain asymptomatic; but under certain stress conditions, EBV may lead to the development of cancers such as Burkitt\u2019s and Hodgkin\u2019s lymphoma and nasopharyngeal carcinoma. EBV\u2010associated cancers result in a large number of deaths in Asian and African population, and no effective cure has still been developed. We, therefore, tried to devise a subunit vaccine with the help of immunoinformatic approaches that can be used for the prevention of EBV\u2010associated malignancies. The epitopes were predicted through B\u2010cell, cytotoxic T lymphocytes (CTL), and helper T lymphocytes (HTL) from the different oncogenic proteins of EBV. A vaccine was designed by combining the B\u2010cell and T\u2010cell (HTL and CTL) epitopes through linkers, and for the enhancement of immunogenicity, an adjuvant was added at the N\u2010terminal. Further, homology modeling was performed to generate the 3D structure of the designed vaccine. Moreover, molecular docking was performed between the designed vaccine and immune receptor (TLR\u20103) to determine the interaction between the final vaccine construct and the immune receptor complex. In addition, molecular dynamics was performed to analyze the stable interactions between the ligand final vaccine model and receptor TLR\u20103 molecule. Lastly, to check the expression of our vaccine construct, we performed in silico cloning. This study needed experimental validation to ensure its effectiveness and potency to control malignancy.<\/jats:p>","DOI":"10.1002\/jcp.27380","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T10:42:28Z","timestamp":1540550548000},"page":"6437-6448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Contriving multiepitope subunit vaccine by exploiting structural and nonstructural viral proteins to prevent Epstein\u2013Barr virus\u2010associated malignancy"],"prefix":"10.1002","volume":"234","author":[{"given":"Rupal","family":"Ojha","sequence":"first","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raj","family":"Nandani","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-0596","authenticated-orcid":false,"given":"Vijay Kumar","family":"Prajapati","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer Rajasthan India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,26]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21495"},{"issue":"3","key":"e_1_2_8_1_3_1","doi-asserted-by":"crossref","first-page":"e00564","DOI":"10.1016\/j.heliyon.2018.e00564","article-title":"Designing and overproducing a tandem epitope of gp350\/220 that shows a potential to become an EBV vaccine","volume":"4","author":"Anyndita N. 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