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Here, we investigated changes in morphology, proliferation, and in vitro differentiation toward mesenchymal and neuronal phenotypes of human DPSCs harvested from differently aged donors. Aging is a physiologic phenomenon occurring with time that hamper body\u2019s capability to maintain homeostasis also affecting the functional reserve. Cytofluorimetric, immunohistochemical, quantitative reverse\u2010transcription polymerase chain reaction (qRT\u2010PCR), and western blot analyses were performed to gain insight for successful regenerative strategies in elderly. We observed a decline in DPSCs proliferation and differentiation potential with age. Interestingly, these cells behaved differently under osteogenic or odontogenic stimuli, showing different age\u2010related mineralization capabilities. Similarly, neurogenic differentiation decreased with age. In conclusion, our observations represent a valid tool for the development of tailored regenerative strategies in an aging society.<\/jats:p>","DOI":"10.1002\/jcp.27472","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T11:22:53Z","timestamp":1540552973000},"page":"7186-7197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Dental pulp stem cells senescence and regenerative potential relationship"],"prefix":"10.1002","volume":"234","author":[{"given":"Iolanda","family":"Iezzi","sequence":"first","affiliation":[{"name":"Department of Clinical and Molecular Sciences\u2010DISCLIMO Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Cerqueni","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Sciences\u2010DISCLIMO Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Licini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Sciences\u2010DISCLIMO Universit\u00e0 Politecnica delle Marche Ancona Italy"},{"name":"Department of Applied Science and Technology (DISAT) Polytechnic of Turin Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guendalina","family":"Lucarini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Sciences\u2010DISCLIMO Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2087-2776","authenticated-orcid":false,"given":"Monica","family":"Mattioli Belmonte","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Sciences\u2010DISCLIMO Universit\u00e0 Politecnica delle Marche Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,26]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0979"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000486592"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(75)80024-0"},{"key":"e_1_2_7_1_5_1","unstructured":"Besdine R. 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