{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T05:14:38Z","timestamp":1768194878398,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T00:00:00Z","timestamp":1540512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The objective of the current study was to explore the role of H19 rs217727 polymorphism in the control of hepatocellular carcinoma (HCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The Student's <jats:italic>t<\/jats:italic> test, Cox regression, and Kaplan\u2013Meier analyses were used to clarify whether the H19 rs217727 polymorphism played an important role in the development of HCC. Real\u2010time polymerase chain reaction (PCR) and western\u2010blot analysis were carried out to measure the levels of H19, microRNA (miR)\u2010675, FAS\u2010associated death domain (FADD), caspase\u20108, and caspase\u20103 among H19 CC, CT, and TT groups, as well as in cells transfected with H19\/si\u2010H19, or miR\u2010675 mimic\/inhibitor. The MTT assay, colony formation assay, and flow cytometry assay were performed to detect the effect of H19\/miR\u2010675 on cell viability, cell colony formation, and cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>T allele of H19 rs217727 polymorphism apparently increased the survival rate of patients with HCC. Meanwhile, H19 enhanced miR\u2010675 expression but reduced the mRNA and protein levels of FADD, caspase\u20103, and caspase\u20108. The T allele of H19 rs217727 polymorphism apparently increased the apoptotic rate of HCC cells. Furthermore, FADD was a virtual target gene of miR\u2010675 with a potential \u201chit\u201d located in the 3\u2032\u2010untranslated region (UTR) of FADD, whereas H19 inhibited FADD expression via increasing the expression of miR\u2010675. Moreover, H19 upregulated the expression of miR\u2010675 whereas reducing the expression of FADD, caspase\u20103, and caspase\u20108. Finally, H19 and miR\u2010675 promoted cell proliferation and cell colony formation but repressed cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In summary, the above findings demonstrated that the polymorphism of rs217727 in H19 was associated with HCC via the H19\/miR\u2010675\/FADD\/caspase\u20108\/caspase\u20103\/apoptosis signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.27485","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T11:19:35Z","timestamp":1540552775000},"page":"7279-7291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Rs217727 polymorphism in H19 promotes cell apoptosis by regulating the expressions of H19 and the activation of its downstream signaling pathway"],"prefix":"10.1002","volume":"234","author":[{"given":"Lili","family":"Ge","sequence":"first","affiliation":[{"name":"Henan Provincial Key Labratory of Children's Genetics and Metabolic Diseases Children's Hospital Affiliated to Zhengzhou University (Henan Children's Hospital, Zhengzhou Children's Hospital) Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinglei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Orthopedics Zhengzhou Orthopedic Hospital Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengnan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Liver Disease Institute of Medical and Pharmaceutical Sciences, Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8994-1437","authenticated-orcid":false,"given":"Xiaoang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Liver Disease Institute of Medical and Pharmaceutical Sciences, Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,26]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2002.5500"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5200619"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"crossref","unstructured":"An integrated encyclopedia of DNA elements in the human genome.Nature2012;489:57\u201074.","DOI":"10.1038\/nature11247"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200956"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12029-014-9659-y"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990812)82:4<490::AID-IJC4>3.0.CO;2-I"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000090831"},{"key":"e_1_2_7_1_10_1","first-page":"3770","article-title":"LncRNA H19 polymorphisms associated with the risk of OSCC in Chinese population","volume":"21","author":"Guo Q. 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